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Gene expression data from NECA (5/-N-ethylcarboxamidoadenosine) treated murine pituitary folliculostellate cells(TtT/GF)

ABSTRACT: Folliculostellate cells constitute as much as 10% of the cells within the anterior pituitary gland; they however do not secrete any of the classical hormones but do express cytokines and growth factors. The endogenous nucleoside adenosine can be either pro- or anti-inflammatory depending on receptor expression and the activation of one or more receptors A1, A2a, A2b and A3. Adenosine stimulates the secretion of immune molecules from folliculostellate; these cells appear to mediate communication between the endocrine and immune systems.

ORGANISM(S): Mus musculus

PROVIDER: GSE15564 | GEO | 2009/04/08

SECONDARY ACCESSION(S): PRJNA115959

REPOSITORIES: GEO

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Gene expression data from NECA (5/-N-ethylcarboxamidoadenosine) treated murine pituitary folliculostellate cells(TtT/GF)

Project description:Folliculostellate cells constitute as much as 10% of the cells within the anterior pituitary gland; they however do not secrete any of the classical hormones but do express cytokines and growth factors. The endogenous nucleoside adenosine can be either pro- or anti-inflammatory depending on receptor expression and the activation of one or more receptors A1, A2a, A2b and A3. Adenosine stimulates the secretion of immune molecules from folliculostellate; these cells appear to mediate communication between the endocrine and immune systems. Replicate (x3) cultures of TtT/GF cells were treated with 10µM NECA (5/-N-ethylcarboxamidoadenosine) for 30 and 120 minutes. RNA was extracted, cRNA prepared and hybridized to murine MG_U74A v2 GeneChip (Affymetrix), changes in expression were compared with untreated controls.

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