Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sevoflurane Exposure Alters Long Non-coding RNA and Messenger RNA Profiles in the Neonatal Mouse Hippocampus

ABSTRACT: The focus of current study is to investigate the effect of developing sevoflurane exposure on lncRNA profiles and molecular signaling involvement in neurotoxicity. An Expression Microarray assay (V3.0) and data analysis services were provided by Arraystar Inc. (Rockville, MD) to assess and compare the global abundance of 27,427 lncRNAs and 18,855 mRNAs in the hippocampi of mice exposed to either sevoflurane or normal air controls.

ORGANISM(S): Mus musculus

PROVIDER: GSE155770 | GEO | 2021/02/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Modeling Alcohol-induced Neurotoxicity using Human Induced Pluripotent Stem Cell-derived Three-Dimensional Cerebral Organoids

Project description:We performed transcriptomic studies using microarray assays and conducted bioinformatics analyses to identify signaling pathways associated with ethanol-induced brain injury in cerebral organoids A human message RNA (mRNA) Expression Microarray assay (V4.0) and data analysis services were provided by Arraystar Inc. (Rockville, MD) to assess and compare the global abundance of 17,195 mRNAs across iPSC-derived 2-month cerebral organoids treated with and without ethanol.

2021-07-13 | GSE154934 | GEO

Comparative study of mRNAs and lncRNAs in endothelial and non-endothelial cells

Project description:To identify lncRNAs enriched in ECs, a microarray was performed to profile ~30,000 lncRNAs and ~26,000 coding transcripts using an Arraystar human LncRNA microarray v3.0 system (Arraystar, Rockville, MD). Three primary human EC lines and two non-EC lines at low passages, namely, human umbilical vein EC (HUVEC), human retinal EC (HREC), human choroidal EC, human dermal fibroblast cell (HDF) and human retinal pigment epithelial (RPE) cell lines, were used in the array. Purity of EC lines was confirmed by acetyl-LDL uptake and EC marker staining

2018-10-08 | GSE105107 | GEO

Long non-coding RNAs in cutaneous squamous cell carcinoma

Project description:The expression of lncRNAs in cutaneous squamous cell carcinoma (cSCC) were determined with a commercially available microarray (Arraystar Inc.,Rockville, Maryland USA). The Arraystar human lncRNA Microarray V3.0 containing 30,586 LncRNAs and 26,109 coding transcripts (mRNAs)

2016-04-13 | GSE74859 | GEO

Sevoflurane increases FKBP5 expression in microglia in the brains of aged marmosets and mice

Project description:Background: Inhalation anesthetics may trigger the hypothalamic–pituitary–adrenal (HPA) axis. FK-506 binding protein (FKBP5) is a critical factor that regulates the HPA axis and is associated with perioperative neurocognitive impairment. However, it is unclear how inhalation anesthetics affects the expression of FKBP5 in different neural cells in the brain. Methods: We used single-nucleus RNA sequencing to characterize hippocampal transcriptome profiles in the brains of aged marmosets and mice after sevoflurane anesthesia. Results: Higher levels of FKBP5 were found in the hippocampi of aged mice after sevoflurane anesthesia. Single nuclear RNA sequencing results from aged mice and marmosets showed that the increased expression of FKBP5 mainly occurred in microglia. The expression of FKBP5 in the hippocampi of aged marmosets and mice increased following long-term exposure to sevoflurane anesthesia. Additionally, the brains of these animals displayed a marked increase in the expression of FKBP5 in microglia after sevoflurane anesthesia. Conclusion: Long-term exposure to sevoflurane augments FKBP5 expression in the hippocampi of aged marmosets and mice, specifically in the microglia.

2024-10-30 | GSE234493 | GEO

sevoflurane exposures on neonatal and adult mice,LC-MSMS

Project description:Sevoflurane is the most commonly used general anesthetic in pediatric surgery, but it has the potential to be neurotoxic. Previous research found that long-term or multiple sevoflurane exposures could cause cognitive deficits in newborn mice but not adult mice, whereas short-term or single inhalations had little effect on cognitive function at both ages. The mechanisms behind these effects, however, are unclear. In the current study, 6- and 60-day-old C57bl mice in the sevoflurane groups were given 3% sevoflurane plus 60% oxygen for three consecutive days, each lasting 2 hours, while those in the control group only got 60% oxygen. The cortex tissues were harvested on the 8th or 62nd day. The tandem mass tags (TMT)pro-based quantitative proteomics combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysi were applied to analyze the influences of multiple sevoflurane anesthesia on the cerebral cortex in mice with various ages. A total of 6247 proteins were measured using the combined quantitative proteomics methods of TMTpro-labeled and LC-MS/MS, 443 of which were associated to the age-dependent neurotoxic mechanism of repeated sevoflurane anesthesia. Our findings would help to further the mechanistic study of age-dependent anesthetic neurotoxicity and contribute to seek for effective protection in the developing brain under general anesthesia.

2022-12-08 | PXD037294 | Pride

Long non-coding RNAs in cutaneous squamous cell carcinoma

2016-04-13 | E-GEOD-74859 | biostudies-arrayexpress

Microarray analysis of gene expression profile in Sjögren’s syndrome patients

Project description:EMT gene expression profile in Sjögren’s syndrome patients were determined by microarray analysis performed by Arraystar Inc. (Rockville, MD) using LncPath™ Human EMT Pathway Microarray (Cat# AS-LP-004H). N=219 potential coding targets related to the EMT signaling pathway were tested.

2021-07-29 | GSE175709 | GEO

Analyses of long non-coding RNAs in human retinal endothelial cells following glucose treatment

Project description:The differential expression of lncRNAs in human retinal endothelial cells (HRECs) was investigated with a commericially available microarray (ArrayStar Human LncRNA Microarray V3.0; ArrayStar Inc., Rockville, Maryland USA). Approximately 28,035 lncRNAs and 21,407 coding transcripts were detected using this microarray. In addition to the array findings, lncRNAs were further constructured using transcriptome databases (including RefSeq, Gencode, UCSC). Sequences were selected using proprietary strategies and each transcript is represented by a specific exon or splice junction probe, which can accurately identify an individual transcript. Nevertheless, both postive and negative probes for housekeeping genes were additionally used as controls.

2018-11-06 | GSE122189 | GEO

Arraystar human lncRNA microarray V3.0

Project description:Arraystar human lncRNA microarray V3.0

| PRJNA760918 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of sevoflurane-treated and sevoflurane-untreated U251 cells Transcriptomes

Project description:To explore the possible mechanisms of Sev inducing ferroptosis in glioma cells, we performed RNA sequencing to screening differential expressed genes between sevoflurane-treated andsevoflurane-treated U251 cells.

2022-01-11 | GSE193295 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data