Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenomic profiling of H3K9me3 in the absence and presence of Periphilin variants

ABSTRACT: Targeted epigenomic profiling method CUT&RUN was used to examine PPHLN-regulated H3K9me3 deposition on chromatin.

ORGANISM(S): Homo sapiens

PROVIDER: GSE155824 | GEO | 2020/08/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Epigenomic profiling of TASOR in Hela cells using CUT&RUN and CUT&Tag

Project description:Targeted epigenomic profiling methods CUT&RUN and CUT&Tag were used to examine TASOR genome binding and TASOR-regulated H3K9me3 deposition on chromatin.

2020-08-05 | GSE155693 | GEO

Epigenomic profiling of H3K9me3 in the absence and presence of Periphilin variants

Project description:Epigenomic profiling of H3K9me3 in the absence and presence of Periphilin variants

| PRJNA655713 | ENA

Uncoupled H3K9me3 nucleation and reinforcement during early zebrafish embryogenesis

Project description:The structural organization of eukaryotic genomes is contingent upon fractionation of DNA into transcriptionally active euchromatin and repressive heterochromatin. However, we still have a limited understanding of how these distinct states are first established during animal embryogenesis. Histone lysine 9 trimethylation (H3K9me3) is critical to heterochromatin formation and bulk establishment of this mark is thought to help drive large-scale remodeling of the initially naive chromatin state during animal embryogenesis. However, a detailed understanding of this process is lacking. Here, we leverage CUT&RUN to define the emerging H3K9me3 landscape of the zebrafish embryo with high sensitivity and temporal resolution. We find that despite the prevalence of DNA transposons in the zebrafish genome, LTR transposons are preferentially targeted for H3K9me3 deposition in the embryo, with different families showing distinct establishment kinetics. High signal-to-noise ratios afforded by CUT&RUN revealed emerging sites of low-amplitude H3K9me3 nucleation prior embryonic genome activation (EGA), with early nucleation primarily at a subset of transposon sequences, loci enriched for maternal piRNAs, and pericentromeres. Unexpectedly, the number of nucleated H3K9me3 sites increases linearly across blastula development while quantitative comparison revealed a >10-fold genome-wide increase in H3K9me3 signal at established sites over just 30 minutes precisely at the onset of major EGA. Later stage analysis revealed continued maturation of the H3K9me3 landscape beyond the initial wave of bulk establishment. Our findings uncover distinct mechanisms of pre- and post-EGA H3K9me3 targeting and reveal decoupling of H3K9me3 establishment from reinforcement during de novo heterochromatin formation.

2024-07-22 | GSE256288 | GEO

Epigenomic profiling of human postmortem cortical samples

Project description:CUT&RUN was used to profile the epigenetic status of cells in human adult neurons (n=3) by measuring enirchment of H3K4me3 and H3K9me3 epigenetic marks over a non-targeting (IgG) control.

2023-02-10 | GSE211871 | GEO

Lamin B1 overexpression alters chromatin organization and gene expression (Cut&Run-seq)

Project description:Overexpression of Lamin B1 leads to the redistribution of H3K9me3 heterochromatin away from the nuclear periphery and into heterochromatic DNA foci within the nucleoplasm. These changes in chromatin organization correlate with misregulated gene expression (mRNA-sequencing), but do not correlate with altered H3K9me3 deposition across the genome (Cut&Run).

2023-04-23 | GSE212108 | GEO

Lamin B1 overexpression alters chromatin organization and gene expression (mRNA-seq)

2023-04-23 | GSE212107 | GEO

h3k9me3-Establishing a reference epigenome in arabidopsis seedlings

Project description:gfe_epigenome - h3k9me3 - Epigenomic mapping - H3K9me3 ChIP-chip 2 dye-swap - chip-chip

2011-04-19 | E-GEOD-24650 | biostudies-arrayexpress

ZNF695 Represses Hepatic Genes and Regulates H3K9me3-Heterochromatin [CUT&RUN]

Project description:ZNF695 Represses Hepatic Genes and Regulates H3K9me3-Heterochromatin [CUT&RUN]

| PRJNA853324 | ENA

CUT&RUN chromatin profiling of H3K4me3, H3K27ac and H3K9me3 histone marks in spermatids and spermatocytes

Project description:Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile changes in chromatin marks between spermatocytes and spermatids, we generated CUT&RUN data of H3K4me3, H3K27ac and H3K9me3 marks in sorted spermatocytes and spermatids.

2019-01-25 | E-MTAB-6932 | biostudies-arrayexpress

h3k9me3-Establishing a reference epigenome in arabidopsis seedlings

Project description:gfe_epigenome - h3k9me3 - Epigenomic mapping - H3K9me3 ChIP-chip

2011-04-19 | GSE24650 | GEO