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RNA-sequencing of skeletal stem cells (SSC) and mesenchymal stem cells (MSC) from hindlimb unloaded and reloaded mice

ABSTRACT: Disuse osteoporosis (DO) occurs in response to mechanical unloading of the skeleton and results in structural changes that compromise bone strength leading to increased fracture risk. Although skeletal stem cells (SSCs) are the main source of osteoblasts and adipocytes in adult bone marrow, whether DO impacts SSC function has not been established. Here, three-month-old male C57BL/6 mice were subjected to a hindlimb unloading (HU) model of disuse osteoporosis for 8 or 14 weeks by tail suspension. Control mice (ambulatory or AMB) were allowed normal use of their hindlimbs. An additional cohort underwent an 8-week recovery period after 8-weeks of unloading. Bone marrow-derived, LepR+ skeletal stem cells (SSCs) were isolated by FACS (n=3 or 4) and pooled prior to RNA isolation. Where indicated, cultured, immuno-depleted, bone marrow-derived mesenchymal stem cells (MSCs) were also collected (n=3) and pooled prior to RNA isolation. Generated libraries were subjected to NGS RNA-seq analysis on the Illumina NextSeq 500. These data delineate unique physiological adaptations to prolonged unloading, demonstrate that DO results in increased leptin expression and sympathetic signaling within the BM niche.

ORGANISM(S): Mus musculus

PROVIDER: GSE156174 | GEO | 2021/12/01

REPOSITORIES: GEO

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