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Adenovirus-mediated E2F-1 gene transfer in melanoma cell line

ABSTRACT: E2F-1 gene expression profiling by adenovirus-mediated gene transfer in SKMEL-2 melanoma cell line. Keywords: other

ORGANISM(S): Homo sapiens

PROVIDER: GSE1562 | GEO | 2005/01/01

SECONDARY ACCESSION(S): PRJNA90877

REPOSITORIES: GEO

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Transcription profiling of human melanoma cell line after Adenovirus-mediated E2F-1 gene transfer

Project description:E2F-1 gene expression profiling by adenovirus-mediated gene transfer in SKMEL-2 melanoma cell line.

2008-06-11 | E-GEOD-1562 | biostudies-arrayexpress

Transcription profiling of human melanoma cell line after Adenovirus-mediated E2F-1 gene transfer

Project description:E2F-1 gene expression profiling by adenovirus-mediated gene transfer in SKMEL-2 melanoma cell line.

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Melanoma cell lines

Project description:5 melanoma cell lines: MelJUSO, A375, 607B, 518A2, Skmel 28 Keywords: other

2004-10-19 | GSE1845 | GEO

Transcription profiling of human SK-MEL-2 melanoma cell line transfected with E2F-1 and doxorubicin or transfected with a LacZ control plus doxorubicin to investigate the molecular mechanisms of a combination of E2F-1 gene therapy and chemotherapy.

Project description:SK-MEL-2 cells treated with Ad-E2F-1 (MOI 2), Ad-E2F-1 (MOI 2)plus doxorubicin 0.1uM, or Ad-LacZ (MOI 2) plus doxorubicin 0.1uM. The combination of E2F-1 gene therapy and chemotherapy produces a synergistic effect on melanoma cell apoptosis. However, the molecular mechanisms have not been fully elucidated. The purpose of this study was to identify novel genes or pathways that may play key roles in apoptosis when E2F-1 gene therapy is combined with doxorubicin chemotherapy. MATERIALS AND METHODS: SK-MEL-2 melanoma cells were infected with Ad-E2F-1 alone, Ad-E2F-1 plus doxorubicin, or Ad-LacZ plus doxorubicin. After 16 hours of treatment, the total RNA was extracted from these cells and subjected to microarray analysis. Quantitative real-time PCR was performed to confirm the microarray data. RESULTS: Our results showed that the combination treatment of Ad-E2F-1 and doxorubicin affected the expression of cytokines, transcription factors, as well as genes involved in signal transduction, cell cycle regulation and apoptosis. CONCLUSION: Our findings have identified, for the first time, novel molecular targets and pathways that led to apoptosis in melanoma cells when Ad-E2F-1 was combined with doxorubicin. The molecular information provided here will enhance further mechanistic studies.

2007-10-27 | E-GEOD-2715 | biostudies-arrayexpress

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Project description:5 melanoma cell lines: MelJUSO, A375, 607B, 518A2, Skmel 28

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Homo sapiens

Project description:Adenovirus-mediated E2F-1 gene transfer in melanoma cell line

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AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis [ChIP-seq]

Project description:In melanoma metastasis, the role of the AP-2alpha transcription factor, which is encoded by TFAP2A, is controversial as some findings have suggested tumor suppressor activity while other studies have shown high TFAP2A expression in node-positive melanoma associated with poor prognosis. Here we demonstrate that AP-2alpha facilitates melanoma metastasis through transcriptional activation of genes within the E2F pathway including EZH2. A BioID screen found that AP-2alpha interacts with members of the nucleosome remodeling and deacetylase (NuRD) complex. Loss of AP-2alpha removed activating chromatin marks in the promoters of EZH2 and other E2F target genes through activation of the NuRD repression complex. In melanoma cells, treatment witj tazemetostat, an FDA-approved and highly specific EZH2 inhibitor, substantially reduced anchorage-independent colony formation and demonstrated heritable anti-metastatic effects, which were dependent on AP-2alpha. Single cell RNA-seq analysis of a metastatic melanoma mouse model revealed hyperexpansion of Tfap2aHigh/E2F activated cell populations in transformed melanoma relative to progenitor melanocyte stem cells. These findings demonstrate that melanoma metastasis is driven by the AP-2alpha/EZH2 pathway and suggest that AP-2alpha expression can be used as a biomarker to predict responsiveness to EZH2 inhibitors for the treatment of advanced melanomas.

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AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis [RNA-seq]

2021-06-29 | GSE162362 | GEO

Screening of new target genes specifically activated by deregulated E2F

Project description:To identify genes specifically activated by deregulated E2F, we examined gene expression profiles using human normal fibroblasts (HFFs), which were starved of serum and re-stimulated with serum, over-expressed with E2F1 or expressed with adenovirus E1a to forcedly inactivate RB. To identify genes activated by growth stimulation, human normal fibroblasts (HFFs) were starved of serum for 48 hr, infected with control virus, restimulated with serum or left serum-starved for 18 hr and harvested. To identify genes actived by deregulated E2F, HFFs were starved of serum for 48 hr, infected with control virus, adenovirus expressing E2F1 or adenovirus E1a, further cultured in the absence of serum for 18 hr and harvested. Gene expression profiles of ecach sample were examined by DNA microarray.

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MicroRNA (miR)-211 loss promotes metabolic vulnerability and BRAF inhibitor sensitivity in melanoma

Project description:We report that miR-211 loss-of-function in the pigmented melanoma cell line SKMEL-28 slowed growth and invasion in vitro, inhibited phosphoinositol-3-kinase (PI3K) signaling, rendered these melanoma cells metabolically vulnerable by attenuating mitochondrial respiration and tricarboxylic acid (TCA) cycling and inhibited melanoma growth in vivo.

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