Dataset Information

RNA-seq analysis in HEK293T WT and SCARNA15-KO cells

ABSTRACT: Small Cajal body-specific RNAs (scaRNA) are conserved small noncoding RNAs that guide post-transcriptional modification of spliceosomal RNA (snRNA), a key step for accurate spliceosome biogenesis and function. Despite scaRNA expression being altered in cancer, how this impacts pre-mRNA splicing remains mostly unexplored. Here, we uncovered SCARNA15 as a critical regulator of cancer-promoting alternative splicing (AS) and stress adaptation. Our scaRNA expression analysis reveals a remarkable SCARNA15 upregulation during malignant transformation, which ensures pseudouridylation (Y) within a functional region of the U2 snRNA. This fines tune AS of distinct transcripts encoding oncoproteins and tumor-suppressors including central p53 regulators. Critically, SCARNA15 loss leads to aberrant p53 hyperactivation and impaired cancer cell survival in response to oxidative stress. Accordingly, SCARNA15-mediated redox homeostasis impacts cancer cell survival, movement and anchorage-independent growth. In sum, these findings highlight a functional role for SCARNA15 and Y in directing cancer-promoting AS programs in human cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156376 | GEO | 2021/07/15

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Small nucleolar RNAs (snoRNAs) and small Cajal body-specific RNAs are non-coding RNAs involved in the maturation of other RNA molecules. Alterations of sno/scaRNA expression may play a role in cancerogenesis. This study elucidates the patterns of sno/scaRNA expression in highly purified cells from 211 chronic lymphocytic leukemia (CLL) patients (Binet stage A) also in comparison with those of different normal B-cell subsets. CLLs display a sno/scaRNAs expression profile similar to normal memory, naÃ¯ve and marginal-zone B-cells, with the exception of a few down-regulated transcripts (SNORA31, -6, -62, and -71C). Our analyses also suggest some heterogeneity in the pattern of sno/scaRNAs expression which is apparently unrelated to the major biological (ZAP-70 and CD38), molecular (IGHV mutation) and cytogenetic markers. Moreover, we found that SNORA70F was significantly down-regulated in poor prognostic subgroups and this phenomenon was associated with the down-regulation of its host gene COBLL1. Finally, we generated an independent model based on SNORA74A and SNORD116-18 expression, which appears to distinguish two different prognostic CLL groups. These data extend the view of sno/scaRNAs deregulation in cancer and may contribute to discover novel biomarkers associated with the disease and potentially useful to predict the clinical outcome of early stage CLL patients. This series of microarray experiments contains the gene expression profiles of purified B-cell chronic lymphocytic leukemia (B-CLL) cells obtained from 211 patients (Binet stage A), 6 normal controls from peripheral blood mononuclear cells (BC), and B-cell sub-populations from tonsils (3 naÃ¯ve B-cells (N), 2 marginal zone (MZ)-like, 3 switched memory (SM) B-cells and 4 germinal center (GC) ). For gene and miRNA expression profiling experiments CLL cells were enriched by negative selection with the EasySep-Human B cell enrichment kit without CD43 depletion (Stem Cell Technologies) using the fully automated protocol of immunomagnetic cell separation with RoboSepTM (Stem Cell Technologies).

Dataset Information

RNA-seq analysis in HEK293T WT and SCARNA15-KO cells

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets