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Two bHLH Transcription Factors, bHLH48 and bHLH60, Associate with PHYTOCHROME INTERACTING FACTOR 7 to Regulate Hypocotyl Elongation in Arabidopsis thaliana

ABSTRACT: A ChIP-Seq was performed using 35S::PIF7-Flash and 35S::bHLH60-Flag transgenic plants after 1 hr shade treatment. Approximately 6374 peaks (5262 genes) were considered as high-confidence of PIF7 binding peaks (Fold change > 2, q-value < 0.05). And 2278 peaks (2143 genes) were considered as high-confidence of bHLH60 binding peaks. The bound genes of PIF7 significantly overlapped with bHLH60-bound genes. And, the transcriptional levels of PIF7 and bHLH60 co-bound genes were also regulated by PIF7 and bHLH48/60. Our results indicated PIF7 and bHLH60 form a heterodimer to bind to promoters of the common target genes and regulate their expressions.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE156584 | GEO | 2021/12/08

REPOSITORIES: GEO

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