Dataset Information

Transcriptome data involved in female Chinese tongue sole (Cynoglossus semilaevis)

ABSTRACT: In the present study, five important somatotropic and reproductive tissues including brain, pituitary gland, liver, gonad and muscle from 6 large females and 6 small males were used for transcriptomic analysis. A mean of 23,389,128 high quality clean reads were obtained from each library and 109, 698, 1,325, 2,299 and 2,141 differentially expressed genes (DEGs) separately recognized from brain, pituitary gland, liver, gonad and muscle between small and large female individuals (fold change ≥ 2 and q < 0.05). These DEGs’ enrichment analysis suggests that the up-regulation of neuroactive ligand-receptor interaction, cell cycle, DNA replication and MAPK signaling pathway in large female group may be involved in the regulation of growth differences.

ORGANISM(S): Cynoglossus semilaevis

PROVIDER: GSE156670 | GEO | 2021/12/31

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background: Salinity is an important abiotic stress that influences the physiological and metabolic activity, reproduction, growth and development of marine fish. It has been suggested that half-smooth tongue sole (Cynoglossus semilaevis), a euryhaline fish species, use a large amount of energy to maintain osmotic pressure balance when exposed to fluctuations in salinity. To delineate the molecular response of C. semilaevis to different levels of salinity, we performed RNA-seq analysis of the liver to identify the genes and molecular and biological processes involved in responding to salinity change. Results: The present study yielded 330.4 million clean reads, of which 83.9% were successfully mapped to the reference genome of C. semilaevis. One hundred twenty-eight differentially expressed genes (DEGs), including 43 up-regulated genes and 85 down-regulated genes, were identified. These DEGs were highly represented in metabolic pathways, steroid biosynthesis, terpenoid backbone biosynthesis, butanoate metabolism, glycerolipid metabolism and the 2-oxocarboxylic acid metabolism pathway. In addition, genes involved in metabolism, osmoregulation and ion transport, signal transduction, immune response and stress response, cytoskeleton remodeling, and apoptosis were affected during acclimation to low salinity. Genes acat2, fdps, hmgcr, hmgcs1, mvk, pmvk, ebp, lss, dhcr7, and dhcr24 were up-regulated and abat, ddc, acy1 were down-regulated in metabolic pathways. Genes aqp10 and slc6a6 were down-regulated in osmoregulation and ion transport. Genes abat, fdps, hmgcs1, mvk, pmvk and dhcr7 were first reported to be associated with salinity adaptation in teleosts. Conclusions: Our results revealed that metabolic pathways, especially lipid metabolism were important for salinity adaptation. The candidate genes identified from this study provide a basis for further studies to investigate the molecular mechanism of salinity adaptation and transcriptional plasticity in marine fish.

Dataset Information

Transcriptome data involved in female Chinese tongue sole (Cynoglossus semilaevis)

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets