Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tracking of CD4+ T cells during CNS autoimmunity

ABSTRACT: Here, we use in vivo labeling at defined anatomical sites for "provance" tracking of immune cells across compartmental borders in the context of experimental autoimmune encephalomyelitis (EAE). Specifically, we labeled T cells in mesenteric or inguinal lymph nodes (LNs) of T cell conditional mitoDendra2 reporter mice via photoconversion at disease onset and re-isolated photoconverted T cells form the LNs, spleen and CNS two days later and we performed scRNA-seq on them. Our experimental system will help to better understand the phenotype and function of T cells migration into the CNS as a consequence of priming in a distinct peripheral immune compartment.

ORGANISM(S): Mus musculus

PROVIDER: GSE156718 | GEO | 2021/04/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in CNS autoimmunity

Project description:We developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model for Multiple Sclerosis are distinct.

2021-03-31 | GSE171122 | GEO

Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity

2021-04-22 | GSE172513 | GEO

Tracking of CD4+ T cells during CNS autoimmunity

Project description:Tracking of CD4+ T cells during CNS autoimmunity

| PRJNA658898 | ENA

Expression data from multiple mouse adipose depots

Project description:Comparing gene expression profiles of murine subcutaneous vs. visceral adipose tissue. Gene expression was analyzed in two subcutaneous depots (inguinal and axillary) and two visceral depots (epididymal and mesenteric) from male C57Bl/6 mice. 4 samples were analyzed as two groups: inguinal and axillary (subcutaneous) and epididymal and mesenteric (visceral). Each sample was derived by pooling RNA from the relevant fat depot from 3 age-matched, male C57Bl6 mice.

2014-01-16 | E-GEOD-53307 | biostudies-arrayexpress

Expression data from CD4+ and CD11c+ cells from DO11.10 RAG2-/- mice

Project description:Abatacept is a recombinant CTLA-4 moleculed fused to a mutated human IgG molecule, which is clinically used in rheumatoid arthritis by inhibiting CD28-costimulation. This study aimed to inverstigate the ability of abatacept -mediated costimulation blockade to induce antigen-specific tolerance during primary immune responses. This is important as some studies have suggested that costimulation blockade can lead to CD4+ T cell anergy which could be beneficial for early therapy of autoimmune diseases such as rheumatoid arthritis. In addition we also investigated the effect that abatacept has on CD11c+ antigen presenting cells. This is important as costimulation blocakde can affect the biderectional interaction between CD4+ T cells and CD11c+ cells influencing the immunological outcome. We used microarrays to identify if abatacept treatment leads to antigen specific anergy using transgenic animals and models of priming and oral tolerance that established a synchronised monoclonal response. In addition this magnified the effect on the CD11c+ antigen presenting cells. This study included 5 experimental groups. DO11.10 RAG2-/- mice have CD4+ T cells specific for the ovalbumin (OVA) peptide OVA323-339. These mice were immunised with CFA/OVA s.c. (primed group) or tolerised by feeding with OVA (50mg/kg) in the drinking water. CD4+ cells were isolated 10 days post immunisation from draining lymph nodes (LNs) of unimmunised (pooled LNs and Spleen), orally tolerised (mesenteric LNs), primed (axillary LNs), primed treated with control IgG (axillary LNs) and primed treated with abatacept (axillary LNs). For CD11c+ cells cells were isolated by pooled secondary lymphoid organs (LNs and spleen).

2014-10-07 | E-GEOD-62089 | biostudies-arrayexpress

Gene expression in PLN vs. ILN or MLN in mice

Project description:Normal gene expression in pancreatic lymph nodes compared to inguinal or mesenteric lymph nodes across different strains of mice (BALB/c, FVB, NOD, NOD.B10).

2009-04-23 | GSE15748 | GEO

Indoles (I3C and DIM)-mediated regulation of microRNAs in DTH model

Project description:Our laboratory observed that indoles (I3C and DIM) suppressed the development of delayed type hypersensitivity (DTH) in mice. Also, we observed differential regulation of regulatory T cells (Tregs) and Th17 cells in mice treated with I3C or DIM. The aim of this experiment was, therefore, to understand the role of I3C or DIM in the regulation of microRNAs (miRs) in immune cells and generation of Tregs and Th17 cells. We also used FICZ, an aryl hydrocarbon receptor (AhR) ligand to understand the role of various ligands in the regulation of Tregs and Th17 cells. To this end, we used draining (inguinal) lymph nodes (LNs) of mice (C57BL/6) with DTH and treated with Vehicle (corn oil, VEH) or I3C or DIM or FICZ. In brief, total RNA including miRs from inguinal LNs were isolated using miRNeasy kit and the protocol of the company was followed (QIAGEN, Valencia, CA). Next, miR arrays were performed at Johns Hopkins University Microarray and Sequencing Core facility using platform.

2019-01-02 | E-MTAB-4076 | biostudies-arrayexpress

Expression data from multiple mouse adipose depots

2014-01-16 | GSE53307 | GEO

In vivo analysis of gene expression changes in mouse astrocytes after traumatic spinal cord injury

Project description:Central nervous system (CNS) lesions become surrounded by neuroprotective borders of newly proliferated reactive astrocytes. Fundamental features of these cells are poorly understood. Here, using temporal transcriptome analysis of Aldh1l1-expressing local astrocytes we showed that after CNS injury, local mature astrocytes dedifferentiated, proliferated, and become transcriptionally reprogrammed to permanently altered new functional states, with persisting downregulation of molecules associated with astrocyte-neuron interactions, and upregulation of molecules associated with wound healing, microbial defence, and interactions with stromal and immune cells. Our findings show that at CNS injury sites, local mature astrocytes proliferate and adopt canonical features of essential wound repair cells that persist in adaptive states and are the predominant source of neuroprotective borders that re-establish CNS integrity by separating neural parenchyma from stromal and immune cells as occurs throughout the healthy CNS.

2023-08-28 | GSE241628 | GEO

Mus musculus

Project description:Sequencing of mesenteric LNs from S. boulardii-treated and naive mice

| PRJNA307494 | ENA