Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of MOLM-13 AML cells following KAT6A CRISPR targeting

ABSTRACT: RNA-seq analysis of MOLM-13 human leukemia cells transduced with lentivirus containing CRISPR-Cas9 EV or sgKAT6A for 5 days

ORGANISM(S): Homo sapiens

PROVIDER: GSE156948 | GEO | 2021/10/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Epigenetic profiling of cells following KAT6A CRISPR targeting

Project description:ChIP-seq analysis of MOLM-13 human leukemia cells transduced with lentivirus containing CRISPR-Cas9 EV or sgKAT6A for 5 days.

2021-10-20 | GSE157041 | GEO

ChIP-seq analysis of ENL following KAT6A CRISPR targeting

Project description:ChIP-seq analysis of ENL in MOLM-13 human leukemia cells transduced with lentivirus containing CRISPR-Cas9 EV or sgKAT6A for 5 days

2021-10-20 | GSE165076 | GEO

ChIP-seq analysis of HA-KAT6A in MOLM-13 AML cells

Project description:ChIP-seq analysis of MOLM-13 human leukemia cells with overexpression of WT KAT6A with HA tag.

2021-10-20 | GSE157039 | GEO

Transcriptional profiling of MOLM-13 AML cells following KAT6A CRISPR targeting

Project description:Transcriptional profiling of MOLM-13 AML cells following KAT6A CRISPR targeting

| PRJNA659646 | ENA

RNA-seq analysis of Molm-13 cell with GADD45g overexpressed

Project description:The growth arrest and DNA-damage induced 45 gamma (GADD45g) is rapidly induced by various physiological and environmental stresses associated with growth arrest. GADD45g has been observed implicated in cell survival, apoptosis, senescence, cell cycle regulation and DNA repair in a variety of human solid tumor types, acting as either tumor promoter or tumor suppressor. To date, the role of GADD45g in hematopoietic malignancies remains completely unknown. Here, we transduced Molm-13 cells with lentiviral vectors expressing doxycycline-inducible GADD45g. Molm-13 cells with dox administration or not were collected for RNA-seq.

2023-06-22 | GSE152932 | GEO

ChIP-seq analysis of HA-KAT6A in MOLM-13 AML cells

Project description:ChIP-seq analysis of HA-KAT6A in MOLM-13 AML cells

| PRJNA659932 | ENA

RNA-seq of MOLM-13 acute myeloid leukemia cell line treated with FIDAS-5

Project description:MOLM-13 acute myeloid leukemia cells were treated with 3 µM FIDAS-5 methionine S-adenosyltransferase 2A (MAT2A) inhibitor or 0.1% DMSO as control for 48 hours

2023-04-22 | E-MTAB-12847 | biostudies-arrayexpress

Validation of CRISPR targeting for proliferation and cytarabine resistance control genes in the acute myeloid leukemia cell line MOLM-13.

Project description:Acute myeloid leukemia patients with FMS-like tyrosine kinase 3-internal tandem duplications and mixed lineage leukemia-protein AF9 fusion proteins suffer from poor clinical outcomes. The MOLM-13 acute myeloid leukemia cell line harbors both of these abnormalities and is used in CRISPR experiments to identify disease drivers. However, experimental observations may be biased or inconclusive in the absence of experimentally validated positive control genes. We validated sgRNAs for knockdown of TP53 for cell proliferation and for DCK knockdown and CDA upregulation for cytarabine resistance control genes in MOLM-13 cells. We have provided a detailed CRISPR protocol applicable to both gene knockdown or activation experiments and downstream leukemic phenotype analyses. Inclusion of these controls in CRISPR experiments will enhance the capacity to identify novel myeloid leukemia drivers in MOLM-13 cells.

| S-EPMC9413368 | biostudies-literature

mRNA expression quantitative Analysis of MOLM-13 and THP-1 treated with I1 compared with control

Project description:The goals of this study are toevaluate the mRNA expressions of genes in MOLM-13 and THP-1 cell lines treated with I13

2022-05-18 | GSE193965 | GEO

mRNA expression quantitative Analysis of MOLM-13 and THP-1 treated with JOA compared with control

Project description:The goals of this study are toevaluate the mRNA expressions of genes in MOLM-13 and THP-1 cell lines treated with JOA

2021-03-25 | GSE167083 | GEO