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Single cell ATAC sequencing profile of a unique mesenchymal progenitor subset from the developing murine forelimb

ABSTRACT: During mouse embryonic development, the limbs emanate from primordial buds of lateral plate mesoderm as proliferating mesenchymal cells cause protrusions of the overlying ectoderm. As the bud expands, several populations of cells are thought to migrate into the emerging limb and contribute to various specialized structures therein. A unique subset has been identified among the migrating populations that contributes to enduring stromal compartments in various tissues and is responsible for aspects of secondary morphogenesis including the formation of entheses throughout the limb. Using a lineage tracing tool, this population was sampled at various stages along the developmental continuum and chromatin accessibility was profiled at the single cell level to determine the fate and function of this cell subtype and the epigenetic programs involved.

ORGANISM(S): Mus musculus

PROVIDER: GSE156950 | GEO | 2022/07/15

REPOSITORIES: GEO

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Single cell RNA sequencing profile of a unique mesenchymal progenitor subset from the developing murine forelimb

Project description:During mouse embryonic development, the limbs emanate from primordial buds of lateral plate mesoderm as proliferating mesenchymal cells cause protrusions of the overlying ectoderm. As the bud expands, several populations of cells are thought to migrate into the emerging limb and contribute to various specialized structures therein. A unique subset has been identified among the migrating populations that contributes to enduring stromal compartments in various tissues and is responsible for aspects of secondary morphogenesis including the formation of entheses throughout the limb. Using a lineage tracing tool, this population was sampled at various stages along the developmental continuum and gene expression was profiled at the single cell level to determine the fate and function of this cell subtype and the genetic programs involved.

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2013-11-15 | E-GEOD-51877 | biostudies-arrayexpress

Identifying an Apical Ectodermal Ridge (AER) signature in embryonic day 11.5 mouse forelimb.

2013-11-15 | GSE51877 | GEO

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Regulation of chondrogenesis in early murine limb mesenchyme by BMP signals

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Project description:The bat offers an alternative paradigm to the standard mouse and chick model of limb development as it has extremely divergent forelimbs (long digits supporting a wing) and hindlimbs (short digits and claws) due the distinct requirements of both aerial and terrestrial locomotion. We used a cross-species microarray approach to identify differentially expressed (DE) genes between the bat (Minniopterus natalensis) forelimb and hindlimb autopods at Carollia developmental stages (CS) 16 and CS17, and between the bat (CS17) and mouse (E13.5) forelimb autopods. Several DE genes were identified, including two homeobox genes, Meis2, a proximal limb-patterning gene, and Hoxd11, a gene involved in digit elongation. Both genes are significantly over-expressed in the developing bat forelimb as compared to the hindlimb and equivalently staged mouse forelimbs.

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