Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Reprogrammed lipid metabolism protects inner nuclear membrane against unsaturated fat

ABSTRACT: Transcriptome analysis of ino4∆ and Mga2∆TM-overexpressing cells in order to understand the mechanism by which cells decide whether to synthesize LDs from the INM or not. A striking difference between the transcriptomes of Mga2∆TM-overexpressing cells (cLDs) and ino4∆ cells (cLDs and nLDs) is the fact that enzymes involved in PL synthesis, for example, the phosphatidylethanolamine methyltransferase CHO2 or the phospholipid methyltransferase OPI3, are downregulated specifically in ino4∆ cells. In contrast, Mga2∆TM overexpressing cells showed an upregulation of the Ole1 desaturase and Mvd1, an enzyme involved in sterol biosynthesis, whereas the expression of PL synthesis factors remained largely unchanged.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE156951 | GEO | 2021/08/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth

Project description:Analysis of gene expression changes in S. cerevisiae in response to OLE1 repression, SPT23 and MGA2 p90 expression, and hypoxic growth

2017-01-31 | GSE94345 | GEO

Disrupted lipid homeostasis in the absence of the transcriptional activator mga2 results in a failure to activate SREBPs in Schizosaccharomyces pombe

Project description:The sre1 pathway senses molecular oxygen in the fission yeast, Schizosaccharomyces pombe. When oxygen becomes limiting, Sre1 is trafficked from the ER to the Golgi where it is cleaved through the actions of the 5-member Dsc E3 ligase complex. The released N-terminus then upregulates genes required for adaptation to hypoxia. Although the sre1 pathway is responsible for regulating a majority of the genes required for hypoxic growth, it is evident that other regulatory genes are involved in this response. To identify novel regulators of adaptation to hypoxia, we screened the S. pombe nonessential haploid deletion collection. This genome wide screen identified nine genes required for production of the membrane-bound transcription factor Sre1N. The characterization of one of these, the S. pombe transcriptional activator mga2, is presented. mga2 is the homolog of SPT23 and MGA2 in S. cerevisiae. In S. cerevisiae SPT23 and MGA2 regulate the transcription of genes involved in lipid metabolism, including the delta-9 fatty acid desaturase, OLE1. We show S. pombe mga2 also regulates lipid metabolism, and that in the absence of mga2, the lipidome is disrupted. Supplementation with unsaturated fatty acids rescued Sre1 cleavage in mga2Δ cells. However, mga2 is not simply required for activation of Sre1 as mga2Δ cells have a normoxia growth defect that is unrelated to Srel activity. Loss of mga2 results in aberrant Dsc1 glycosylation, suggesting that the Dsc complex is not properly localized to the Golgi. These results establish unsaturated fatty acids as a regulator of the SREBP pathway in fission yeast.

2016-04-06 | GSE60544 | GEO

Adipose-derived mesenchymal stem cells overexpressing thrombomodulin reduce thrombogenic risk and enhance therapeutic efficacy

Project description:Adipose-derived mesenchymal stem cells (ASCs) are expected to be a new therapy to prevent progression of renal fibrosis; however, they were reported to have procoagulant activity, raising concerns about thrombogenic risk. Thrombomodulin (TM) acts as an anticoagulant factor by binding to thrombin, leading to the hypothesis that ASCs overexpressing TM (TM-ASCs) could be safely used for cell therapy by reducing the risk of thromboembolism. Overexpression of TM using adeno-associated virus (AAV) reduced rat deaths related to renal infarction and pulmonary embolism induced by ASCs. Interestingly, TM-ASCs alleviated renal inflammation and fibrosis potently in ischemia-reperfusion injury (IRI) rats. The therapeutic mechanism of TM-ASCs involved increased prostaglandin E2 (PGE2) secretion, a shift in the macrophage phenotype to immunosuppressive M2 in vitro, and potent M2 macrophage polarization in the damaged areas in vivo. We produced ASCs with enhanced TM expression by pretreatment with a selective inhibitory kappa-B kinase-β (IKKβ) inhibitor. These ASCs also improved thrombotic mortality and markedly attenuated renal fibrosis induced by IRI, similar to TM-ASCs generated by AAV. ASCs with enhanced TM expression by IKKβ also increased PGE2 secretion, leading to a more pronounced polarization of M2 macrophages. Taken together, ASCs with enhanced TM expression eliminated the risk of thrombosis and reinforced therapeutic efficacy. These ASCs might become a standard cellular therapy for renal fibrosis via the intravascular injection of mesenchymal stem cells in the future.

2024-09-24 | GSE277567 | GEO

RNA-seq analysis of fission yeast mga2∆ and mga2∆ cbf11∆ mutants in standard and ammonium-supplemented complex media

Project description:Transcriptome analyses of the fission yeast Schizosaccharomyces pombe cells lacking the CSL transcription factor cbf11 and/or mga2. Both genotypes were analyzed when grown to exponential phase in the complex YES medium, or in YES supplemented with ammonium chloride. The aim was to identify genes regulated by Mga2 and/or Cbf11, and how mutant phenotypes are affected by the presence of a good nitrogen source.

2024-04-05 | E-MTAB-13303 | biostudies-arrayexpress

Project description:OLE1 inhibitor resistant mutants

| PRJNA496936 | ENA

Mitochondrial 12S hypermethylation in HeLa cells and A1555G cybrids

2012-09-18 | GSE33780 | GEO

RNA-seq analysis of HeLa cells overexpressing histone methyltransferase KMT2B

Project description:RNA-seq analysis of HeLa cells overexpressing histone methyltransferase KMT2B

| PRJNA862962 | ENA

Mitochondrial 12S hypermethylation in HeLa cells and A1555G cybrids

Project description:This dataset investigates the transcriptional effect of mitochondrial 12S rRNA hypermethylation, both by overexpressing the mitochondrial methyltransferase mtTFB1 in HeLa cells and by using A1555G cybrids, where the 12S rRNA is hypermethylated. HeLa cells overexpressing a methyltransferase-deficient mtTFB1 (mtTFB1[G65A]) and wild-type A1555A cybrids were used as controls. four samples with 12S rRNA hypermethylation (two cell lines, with two biological replicates each) versus four samples with basal 12S rRNA methylation (two cell lines, with two biological replicates each)

2012-09-18 | E-GEOD-33780 | biostudies-arrayexpress

Inducible repositioning of genes to the inner nuclear membrane

Project description:Nuclear compartmentalization appears to play an important role in regulating metazoan genes. While studies on immunoglobulin (Ig) and other loci have correlated positioning at the nuclear lamina with gene repression, the functional consequences of this compartmentalization remain untested. We devised an approach for inducible tethering of genes to the inner nuclear membrane (INM) and demonstrate with 3D DNA-ImmunoFISH, repositioning of chromosomal regions to the nuclear lamina. Relocalization requires mitotic nuclear envelope breakdown and reformation. Tethering leads to the accumulation of lamin and INM proteins but not to association with pericentromeric heterochromatin or nuclear pore complexes. Recruitment of genes to the INM can result in their transcriptional repression. Using DamID we show that as is the case for our model system, inactive Ig loci at the nuclear periphery are contacted by INM and lamina components. We propose that such molecular interactions are used to compartmentalize and limit the accessibility of Ig loci. Keywords: Inducible

2008-01-16 | GSE10176 | GEO

Immune gene expression analysis for oral leukplakia samples

Project description:Oral leukoplakia is common and may in some cases progress to carcinoma. Proliferative leukoplakia (PL) is a progressive, often multifocal subtype with a high rate of malignant transformation (MT) compared to the more common localized leukoplakia (LL). We hypothesized that the immune microenvironment and gene expression patterns would be distinct for PL compared to LL. We summarize key clinicopathologic features among PL and LL and compare cancer-free survival (CFS) between subgroups. We analyze immunologic gene expression profiling (GEP) in PL and LL tissue samples (NanoString PanCancer Immune Oncology Profiling). We integrate immune cell activation and spatial distribution patterns in tissue samples using multiplexed immunofluorescence and digital image capture to further define PL and LL. Among N=58 patients (PL: 29, LL: 29), only the clinical diagnosis of PL was associated with significantly decreased CFS (HR 11.25, p<0.01); 5-year CFS 46.8% and 83.6% among PL and LL patients, respectively. CD8+ T cells and Tregs were more abundant among PL samples (p<0.01) regardless of degree of epithelial dysplasia, and often colocalized to the dysplasia-stromal interface. Gene set analysis identified granzyme-M (GZMM) as the most differentially expressed gene favoring the PL subgroup (log2 fold change 1.93, adjusted p<0.001). PD-L1 was comparatively over-expressed among PL samples, with higher (>5) PD-L1 scores predicting worse CFS (p<0.01). PL predicts a high rate of MT within 5-years of diagnosis. Robust CD8+ T cell and Treg signature along with relative PD-L1 over-expression compared with LL provides strong rationale for PD-1/L1 axis blockade using preventative immunotherapy.

2021-10-15 | GSE184944 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data