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CRISPR and biochemical screens identify MAZ as a co-factor in CTCF-mediated insulation at Hox clusters

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus synthetic construct

PROVIDER: GSE157139 | GEO | 2022/02/06

REPOSITORIES: GEO

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CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters.

Project description:CCCTC-binding factor (CTCF) is critical to three-dimensional genome organization. Upon differentiation, CTCF insulates active and repressed genes within Hox gene clusters. We conducted a genome-wide CRISPR knockout (KO) screen to identify genes required for CTCF-boundary activity at the HoxA cluster, complemented by biochemical approaches. Among the candidates, we identified Myc-associated zinc-finger protein (MAZ) as a cofactor in CTCF insulation. MAZ colocalizes with CTCF at chromatin borders and, similar to CTCF, interacts with the cohesin subunit RAD21. MAZ KO disrupts gene expression and local contacts within topologically associating domains. Similar to CTCF motif deletions, MAZ motif deletions lead to derepression of posterior Hox genes immediately after CTCF boundaries upon differentiation, giving rise to homeotic transformations in mouse. Thus, MAZ is a factor contributing to appropriate insulation, gene expression and genomic architecture during development.

| S-EPMC8837555 | biostudies-literature

CRISPR and biochemical screens identify MAZ as a co-factor in CTCF-mediated insulation at Hox clusters [MAZ KO]

Project description:In this experiment, we sought to analyze how transcriptome of WT and MAZ knock-out (KO) cells change during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs)

2022-02-06 | GSE157137 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters

Project description:In this experiment, we sought to identify how the distribution of MAZ change in WT, MAZ HoxA(Δ5|6), and MAZ HoxD(Δ4|8) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs)

2022-02-06 | GSE190991 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [CRISPR_set8]

Project description:In this experiment, we sought to analyze the genes positively or negatively selected during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) with particular focus on the genes enriched for CTCF boundary disruption at HoxA cluster

2022-02-06 | GSE192435 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [CRISPR_set9]

2022-02-06 | GSE192437 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set7]

Project description:In this experiment, we sought to identify the distribution of RAD21 in cervical motor neurons (MNs) that is formed through in vitro differentiation of mESCs.

2022-02-06 | GSE192430 | GEO

CRISPR and biochemical screens identify MAZ as a co-factor in CTCF-mediated insulation at Hox clusters [Hi-C]

Project description:In this experiment, we sought to analyze how three dimensional genome organization of WT and MAZ knock-out (KO) cells change during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs)

2022-02-06 | GSE157136 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set6]

Project description:In this experiment, we sought to identify how the distribution of CTCF and MAZ change in WT and MAZ knock-out (KO) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs)

2022-02-06 | GSE192426 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set4]

Project description:In this experiment, we sought to identify how the distribution of H3K27me3 and H3K4me3 change in WT, MAZ HoxA(delta5|6), MAZ HoxD(delta4|8), and CTCF (delta5|6:6|7) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs)

2022-02-06 | GSE190990 | GEO

CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set3]

Project description:In this experiment, we sought to identify how the distribution of CTCF change in WT, MAZ HoxA(Δ5|6), MAZ HoxD(Δ4|8), and CTCF (Δ5|6:6|7) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs).

2022-02-06 | GSE190989 | GEO

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