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Transcriptome analysis of time couse of leafy spurge infection with Xam

ABSTRACT: The goal of this study was to identify signaling processes associated with infection and recovery of leafy spurge inoculated with Xanthomonas axonopodis pv. manihotis (Xam).

ORGANISM(S): Manihot esculenta Euphorbia esula

PROVIDER: GSE15751 | GEO | 2009/08/15

SECONDARY ACCESSION(S): PRJNA116775

REPOSITORIES: GEO

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Transcriptome analysis of time couse of leafy spurge infection with Xam

Project description:The goal of this study was to identify signaling processes associated with infection and recovery of leafy spurge inoculated with Xanthomonas axonopodis pv. manihotis (Xam). Two dye-swap technical replicates for each of 3 biological replicates for each treatment were analyzed. A time course analysis of Xam infected and mock inoculated leafy spurge was performed. Time points were 1, 7 and 21 days after inoculation (or mock inoculation). Thus, 36 labeled samples were hybridized in a 2 dye rolling circle hybridization scheme to 18 arrays.

2010-06-24 | E-GEOD-15751 | biostudies-arrayexpress

Expression analysis of leafy spurge crown buds during dormancy transitions and release.

Project description:This submission contains the RNAseq data from one (just the study on paradormancy release) of several studies used to assemble the leafy spurge tranacriptome. These assembled transcrptome was built from 1) A study of leafy spurge crown buds through a time course for paradormancy release induced by excision of the aerial portion of the shoot (this study). 2) A study of leafy spurge crown buds through a seasonal dormancy time course where buds transitioned from paradoprmancy to endodormancy and then to ecodormancy (Series GSE71321). 3) A study identical to #2 above but where leafy spurge plants were first treated with glyphosate (Series GSE71406). 4) A previously submitted study (Series GSE56509) of leafy spurge shoots following treament with glyphosate was also used to assemble the transcriptome.

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Expression analysis of leafy spurge crown buds during seasonal dormancy transitions.

Project description:This submission contains the RNAseq data from a study of leafy spurge crown buds transitioning through a seasonal dormancy time course where buds transitioned from paradoprmancy to endodormancy and then to ecodormancy. The sequences in this study were mapped to an assembled transcriptome built from sequences from this study along with sequences from : 1) A study of leafy spurge crown buds through a time course for paradormancy release induced by excision of the aerial portion of the shoot (Series GSE71317). 2) A study identical to this study but where leafy spurge plants were first treated with glyphosate (Series GSE71406). 3) A previously submitted study of leafy spurge shoots following treatment with glyphosate was also used to assemble the transcriptome (Series GSE56509).

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loss of paradormancy in underground adventitious buds of leafy spurge (Euphorbia esula)

Project description:This study is a time course of growth induction (0, 12, 24, 48, and 72 hrs) following the breaking of paradormancy in underground buds of the perennial weed leafy spurge (Euphorbia esula). Keywords: Time course, growth induction, apical dominance, paradormancy, root buds, leafy spurge

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Expression analysis of leafy spurge crown buds during seasonal dormancy transitions after glyphosate treatments.

Project description:This submission contains the RNAseq data from a study of leafy spurge crown buds transitioning through a seasonal dormancy time course following glyphosate treatments where buds transitioned from paradoprmancy to endodormancy and then to ecodormancy. The sequences in this study were mapped to an assembled transcriptome built from sequences from this study along with sequences from : 1) A study of leafy spurge crown buds through a time course for paradormancy release induced by excision of the aerial portion of the shoot (Series GSE71317). 2) A study identical to this study but where leafy spurge plants were not treated with glyphosate (Series GSE71321). 3) A previously submitted study of leafy spurge shoots following treament with glyphosate was also used to assemble the transcriptome (Series GSE56509).

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Germination of Leafy Spurge (Euphorbia esula) Seeds under Constant and Alternating Temperature

Project description:Transcriptome changes were investigated for Euphorbia esula (leafy spurge) seeds with a focus on the effect of constant and diurnal fluctuating temperature on dormancy and germination. Leafy spurge seeds do not germinate when incubated for 21 days at 20°C constant temperatures, but nearly 30% germinate after 21 days under fluctuating temperatures 20:30°C (16:8 h). Incubation at 20°C for 21 followed by 20:30°C resulted in approximately 63% germination in about 10 days. A cDNA microarray representing approximately 22,000 unique sequences was used to profile transcriptome changes.

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Seasonal variation in leafy spurge crown bud gene expression

Project description:These experiments were designed to follow the seasonal changes in the transcriptome of field-grown leafy spurge crown buds. The goal was to identify specific signaling pathways and marker genes characteristic to the changes that occur when the buds transitioned from paradormancy through endodormancy and into ecodormancy. Keywords: Time course seasonal dormancy

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Expression analysis of leafy spurge crown buds during seasonal dormancy transitions after glyphosate treatments.

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