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Effect of 8-oxoguanine DNA glycosylase 1 (OGG1) inhibitor on pro-inflammatory gene expression.

ABSTRACT: Balbc J mouse received vehicle (solvent) or OGG1 inhibitor 1, 4, 8, 12, 24, 28 , 32, 36, 40 and 44h after RSV infection via intraperitoneal route. RSV (10^6 PFU) was installed into lungs via intranasal route in 60 microliters of solvent). One, 2, 3, 6, 12, 24, 48 and 72 hour after RSV challenge mice were sacrificed, lungs were removed and RNAs were isolated from individual lungs (n=6) After reverse transcription cDNAs were pooled and changes in gene expression was determined by using SABiosciences Mouse Inflammatory Cytokines & Receptors PCR Array (PAMM-011ZD)

ORGANISM(S): Mus musculus

PROVIDER: GSE157630 | GEO | 2021/09/14

REPOSITORIES: GEO

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Effect of 8-oxoguanine DNA glycosylase 1 (OGG1) inhibitor on pro-inflammatory gene expression.

Project description:Balbc J mouse received vehicle (solvent) or OGG1 inhibitor 1 h prior to treatment via intraperitoneal route. Tumor necrosis factor alpha (TNFa, 20 ng per ml) was installed into lungs via intranasal route in 60 microliters of solvent). One hour after TNFa challenge mice were sacrificed, lungs were removed and RNAs were isolated from individual lungs (n=6) After reverse transcription cDNAs were pooled and changes in gene expression was determined by using SABiosciences Mouse Inflammatory Cytokines & Receptors PCR Array (PAMM-011Z)

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Real-time quantitative PCR analysis of mouse lungs

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