Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

FKBP4 knockdown effect on the gene expression of H1975 cells

ABSTRACT: H1975 cells were transfected with FKBP4 targeting shRNA and the control shRNA, then mRNA were extracted for high-throughput sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE157826 | GEO | 2020/09/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

FKBP4 knockdown effect on the gene expression of H1975 cells

Project description:FKBP4 knockdown effect on the gene expression of H1975 cells

| PRJNA662922 | ENA

Gene expression profile of cell lines 2106T, H1975 and MeWo after knockdown of PAEP.

Project description:Gene expression profile was analyzed after knockdown of PAEP in lung cancer cell lines 2106T and H1975 as well as in skin cancer cell line MeWo. The aim of the study was to investigate the role of PAEP and its protein product glycodelin in lung cancer cell lines. Cells were treated with a control siRNA or a siRNA pool of 4 siRNAs targeting PAEP. RNA of three biological replicates each was extracted and used for Affymetrix analyses.

2015-04-22 | E-GEOD-60888 | biostudies-arrayexpress

Effect of YBX1 knockdown on gene expression of differentiated C3H10T1/2 brown adipocytes

Project description:To investigate the function of YBX1 in the regulation of brown adipose aging, differentiated C3H10T1/2 brown adipocytes were transfected with specific siRNAs targeting YBX1 or control siRNA. Total RNA was extracted at 48h after transfection to performed gene expression profiling analysis by high throughput RNA-seq.

2024-01-04 | GSE222103 | GEO

Transcriptome profiling of Anlotinib-resistant NCI-H1975 and Anlotinib-treated Anlotinib-resistant NCI-H1975

Project description:Further to our previous study (E-MTAB-5997), here we performed transcriptome profiling on Anlotinib-resistant NCI-H1975 and Anlotinib-treated Anlotinib-resistant NCI-H1975, and would like to understand the effects of Anlotinib on Anlotinib-resistant NCI-H1975 cell, compare the different transcriptome profiling on NCI-H1975 cells and Anlotinib-resistant NCI-H1975 cells, sought to find the biomarker for explaining Anlotinib resistance.

2019-07-01 | E-MTAB-7068 | biostudies-arrayexpress

Comparative proteomics of CTS effect on human lung cancer H1975

Project description:Cryptotanshinone (CTS) is a lipophilic constituent of Salvia miltiorrhiza, with a broad-spectrum anticancer activity. We have observed that CTS enhances the efficacy of gefitinib in human lung cancer H1975 cells, yet little is known about its molecular mechanism. To explore how CTS enhances H1975 cell sensitivity to gefitinib, we figured out differential proteins of H1975 cells treated by gefitinib alone or in combination with CTS using label-free liquid chromatography-mass spectrometry (LC-MS). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) bioinformatic analyses of the differential proteins were performed. CTS enhanced H1975 cell sensitivity to gefitinib in vitro and in vivo, with 115 and 128 differential proteins identified respectively. GO enrichment, KEGG analysis, and PPI network comprehensively demonstrated that CTS mainly impacted the redox process and fatty acid metabolism in H1975 cells. Moreover, three differential proteins were validated by RT-qPCR and Western blot. In conclusion, we used a proteomic method to study the mechanism of CTS enhancing gefitinib sensitivity in H1975 cells. Our finding reveals the potential protein targets of CTS in overcoming gefitinib resistance which may be therapeutical targets in lung cancer.

2022-02-23 | PXD031533 | Pride

Homo sapiens strain:H1975

Project description:H1975 --0915c

| PRJNA821452 | ENA

Erastin induced protein expression change in H1975 cells

Project description:H1975 cells were treated with DMSO and erastin, respectively. Protein was extracted and separated using SDS-PAGE electrophoresis. MS analysis was performed and protein was identified and quantified using Proteome Discoverer™ 1.3 software using the SEQUEST® search engine.

2021-09-09 | PXD020205 | Pride

Effect of Pirfenidone on NSCLC [A549/H1975 0.75 mg/ml Pirf]

Project description:Investigating the effect of Pirfenidone on NSCLC cells Although immune checkpoint and targeted therapies offer remarkable benefits for lung cancer treatment, some patients do not qualify for these regimens or do not exhibit consistent benefit. Provided that lung cancer appears to be driven by transforming growth factor beta signaling, we investigated the single drug potency of Pirfenidone, an approved drug for the treatment of lung fibrosis which is known to inhibit TGFB1 mRNA. Human A549 and H1975 lung adenocarcinoma cell lines were stimulated for 6h and 25h with 0.75 mg/ml or 1.5 mg/ml Pirfenidone or the respective volume of PBS as vehicle control. Total RNA was extracted and subjected to transcriptome analysis by microarrays.

2019-12-17 | GSE136932 | GEO

Gene expression profile of cell lines 2106T, H1975 and MeWo after knockdown of PAEP.

2015-04-22 | GSE60888 | GEO

Analysis of gene expression signatures for RAB27A knockdown

Project description:To comprehensively detect differential gene expression between the two ovarian cancer cell lines (ES-2 and SKOV3ip1) transfected with control shRNA or shRAB27A, total RNA was extracted and cDNA microarray analyses were performed.

2021-05-10 | GSE159572 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data