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Effect of DDX5/DDX17 on genome-wide RNA Polymerase II distribution in SH-SY5Y cells

ABSTRACT: This project includes ChIP of total RNA pol II in human SH-SY5Y neuroblastoma cells following the joint depletion of RNA helicases DDX5 and DDX17 to determine the genome-wide effect of DDX5/DDX17 on RNA Pol II distribution.

ORGANISM(S): Homo sapiens

PROVIDER: GSE157850 | GEO | 2022/09/21

REPOSITORIES: GEO

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Downregulation of DDX5/DDX17 and REST

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Effect of DDX5/DDX17 on genome-wide RNA Polymerase II distribution in SH-SY5Y cells

Project description:Effect of DDX5/DDX17 on genome-wide RNA Polymerase II distribution in SH-SY5Y cells

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Project description:DEAD-box RNA helicases DDX5 and DDX17 regulate gene expression at different levels such as transcription and splicing, but the underlying mechanisms are not fully understood. A transcriptome analysis of DDX5/DDX17-depleted human cells confirmed was carried out to further characterize the widespread deregulation of alternative splicing. This study also revealed a deregulation of 3’ end processing leading to transcriptional read-through. Univ Lyon, ENS de Lyon, CNRS UMR 5239, INSERM U1293, Laboratory of Biology and Modelling of the Cell, 46 Allée d'Italie, F-69007, Lyon, France. Corresponding author: cyril.bourgeois@inserm.fr

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