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Factors affecting the rapid changes of protein under short-term heat stress

ABSTRACT: The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are still unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features implicated in protein translation and degradation on protein expression were evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly affect protein expression, and their combined contribution explains 58.2% and 66.4% of protein abundance variation in two types of heat stresses. Of which, transcription contributes more to the protein expression under severe heat stress (31%) than to mild stress (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress. This study revealed the main factors affecting the changes of protein abundance in the early stage of heat stress, and explained the mystery that plants can express protein rapidly under heat stress.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE157909 | GEO | 2021/03/23

REPOSITORIES: GEO

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The temperature quickly modulates protein expression by Codon Usage in wheat filling grain

Project description:The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features which implicated in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly drive the changes in protein expression under heat stress, and their combined contribution explains 58.2% and 66.4% of protein variation in 30 and 40 °C, respectively. Of which, transcription contributes more to the alteration in protein content under 40 °C (31%) than to 30 °C (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress.

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