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Role of RUVBL2 in LPS-induced proinflammatory gene expression of RAW264.7 cells

ABSTRACT: The aim of this experiment is to compare gene expression proflies of RAW264.7 cells trasnfected with non-targeting siRNA or siRNA against RUVBL2, in the presence or absence of LPS.

ORGANISM(S): Mus musculus

PROVIDER: GSE158135 | GEO | 2021/05/31

REPOSITORIES: GEO

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Role of RUVBL2 in LPS-induced proinflammatory gene expression of RAW264.7 cells

Project description:Role of RUVBL2 in LPS-induced proinflammatory gene expression of RAW264.7 cells

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Project description:Low-intensity pulsed ultrasound (LIPUS) is a special type of low intensity ultrasound. In periodontal disease, LIPUS was applied as an adjuvant and non-invasive therapeutic treatment. While, the specific mechanism of LIPUS in the treatment of periodontal disease is not quite clear.RAW264.7 cells were induced to M1/M2 macrophage-like polarization by LPS/IL4. LIPUS was performed to stimulate RAW264.7 cells at an intensity of 45 mW/cm2, 25 min, interval 24 h, twice. The polyA mRNA sequencing of LPS induced RAW264.7 cells, LPS induced and LIPUS treated RAW264.7 cells were conducted.Our results suggested that LIPUS played an anti-inflammatory role by inhibiting LPS-induced M1 polarization of RAW264.7 cells in an Wnt2b/AXIN/β-catenin dependent way. LIPUS may inhibit inflammation in periodontal diseases by regulating macrophage differentiation, so as to play a therapeutic role in periodontal diseases.

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