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ChIP-seq of CREPT in intestinal crypts

ABSTRACT: CREPT was demonstrated as a key regulator of intestinal stem cells. To figure out the role of CREPT on transcription level, a ChIP-seq experiment was performed to identify the CREPT down-stream genes in intestinal crypt cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE158243 | GEO | 2020/11/08

REPOSITORIES: GEO

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ChIP-seq of CREPT in intestinal crypts

Project description:ChIP-seq of CREPT in intestinal crypts

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RNA-seq of crypt and villus cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type controls

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Gene expression profiles of WT and CREPT deleted Lgr5+ intestinal stem cells

Project description:Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPT in ISCs, we isolated WT and CREPT deleted Lgr5+ ISCs (Lgr5-CREPTKO) to perform Next generation sequencing.

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RNA-seq of crypt cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type crypts

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PTEN-deficient intestinal stem cells initiate intestinal polyposis

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