Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA base-editing activity, functionality and specificity

ABSTRACT: We use RNA-seq to study the transcriptome wide specificity profiles of A-to-I RNA editing by split-ADAR2 constructs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE158656 | GEO | 2022/01/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA base-editing activity, functionality and specificity

Project description:Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA base-editing activity, functionality and specificity

| PRJNA666136 | ENA

Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA editing activity and specificity.

Project description:Adenosine deaminases acting on RNA (ADARs) can be repurposed to enable programmable RNA editing, however their exogenous delivery leads to transcriptome-wide off-targeting, and additionally, enzymatic activity on certain RNA motifs, especially those flanked by a 5' guanosine is very low thus limiting their utility as a transcriptome engineering toolset. Towards addressing these issues, we first performed a novel deep mutational scan of the ADAR2 deaminase domain, directly measuring the impact of every amino acid substitution across 261 residues, on RNA editing. This enabled us to create a domain-wide mutagenesis map while also revealing a novel hyperactive variant with improved enzymatic activity at 5'-GAN-3' motifs. As overexpression of ADAR enzymes, especially hyperactive variants, can lead to significant transcriptome-wide off-targeting, we next engineered a split-ADAR2 deaminase which resulted in >100-fold more specific RNA editing as compared to full-length deaminase overexpression. Taken together, we anticipate this systematic engineering of the ADAR2 deaminase domain will enable broader utility of the ADAR toolset for RNA biotechnology applications.

| S-EPMC8809894 | biostudies-literature

Overexpression of RNA deaminase ADAR2 in Th17 cells

Project description:ADAR2 is induced upon T cell activation and its immunological function is not known. Here, we performed RNA-seq on control and Th17 cells overexpressing wildtype or catalytic dead ADAR2 to identify A-to-I edited transcripts that are targets of ADAR2.

2024-12-31 | GSE227153 | GEO

Knockdown of RNA deaminase ADAR2 in Th17 cells

Project description:ADAR2 is induced upon T cell activation and its immunological function is not known. Here, we performed RNA-seq on Cas9+ control and Th17 cells expressing the PSIN empty vector control or guide RNAs against the ATG start codon or ADAR2 to identify ADAR2 dependent A-to-I edited transcripts.

2024-12-31 | GSE227152 | GEO

MiRNAinU118_Cells_Inactive_vs_ active_ Adar2

Project description:MicroRNA expression profile of NB U118 cells transfected with a plasmid directing an inactive Adar2 vs an active Adar2 - 3 biological replicates 1 Condition experiment, 3 biological replicates, 2 replicates/array

2015-02-28 | E-GEOD-63694 | biostudies-arrayexpress

MiRNAinU118_Cells_Inactive_vs_ active_ Adar2

Project description:MicroRNA expression profile of NB U118 cells transfected with a plasmid directing an inactive Adar2 vs an active Adar2 - 3 biological replicates

2015-02-28 | GSE63694 | GEO

Overexpression of RNA deaminase ADAR2 in Th17 cells

Project description:Overexpression of RNA deaminase ADAR2 in Th17 cells

| PRJNA943423 | ENA

Knockdown of RNA deaminase ADAR2 in Th17 cells

Project description:Knockdown of RNA deaminase ADAR2 in Th17 cells

| PRJNA943424 | ENA

Changes in gene expression and RNA editing in Adar2-WT and Adar2-KO Mouse embryonic Fibroblasts (MEFs)

Project description:Purpose: Measure gene expression and RNA editing changes in Adar-WT and Adar2-KO MEFs Methods: Performed PolyA+ RNA-seq (single end, one sample each) of Adar2-WT and Adar2-KO MEFs Results: Observed changes in editing and gene expression of several genes Conclusion: Adar2 influences gene expression and editing of transcripts

2016-12-23 | GSE74746 | GEO

ADAR2 in HEK293T cells

Project description:ADAR2 in HEK293T cells

| PRJNA422795 | ENA