Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq analysis of genes activated 90 minutes, or 7 days post irradiation or untreated cells with knockdown of NFKBIA

ABSTRACT: Goal of the study was to investigate genes regulated differentially after 90 minutes versus 7 days after irradiation. 7 day time point corresponds to cellular senescence. U2OS cells were irradiated with 20 Gy and harvested either 90 minutes or 7 days. In addition cells were left untreated (control) or had knockdown of NFKBIA (protein name Ikappa B alpha). Ikappa B alpha is the main inhibitor of the transcription factor NFkappa B. NFkappa B regulates transcription of many factors constituting the SASP (senescence associated secretory phenotype).

ORGANISM(S): Homo sapiens

PROVIDER: GSE158743 | GEO | 2020/09/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNAseq analysis of genes activated 90 minutes, or 7 days post irradiation or untreated cells with knockdown of NFKBIA

Project description:RNAseq analysis of genes activated 90 minutes, or 7 days post irradiation or untreated cells with knockdown of NFKBIA

| PRJNA666405 | ENA

ATAC-seq in GR18 cell line comparing cells treated for 90 minutes with 1 micro molar dexamethasone with vehicle (ethanol) control-treated cells

Project description:We performed ATAC-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment for 90 minutes.

2019-03-11 | E-MTAB-7746 | biostudies-arrayexpress

3dpf untreated zebrafish larvae vs. 3dpf zebrafish larvae treated with 20mM PTZ for 30 minutes and 90 minutes

Project description:Transcriptional profiling of 3dpf wild type zebrafish larvae treated with 20mM PTZ for 30 and 90 minutes compared with 3dpf wild type control untreated zebrafish larvae.

2017-02-24 | GSE95312 | GEO

mRNA sequencing analysis of transcripts from CA1 regions 90 minutes after Long-Term Potentiation was induced

Project description:Purpose: Study the differentially expressed genes in hippocampal rat CA1 regions 90 minutes after Long-Term Potentiation (LTP) was induced using field recordings Methods: hippocampal rat CA1 region mRNA profiles of control (unstimulated) and 90 minutes after LTPwas induced (LTP 90) were generated by deep sequencing. A pool of approximately 10 CA1 regions collected from at least 3 different animals were used. The seq library was prepared using TruSeq RNA Sample Prep Kit v2 protocol (Illumina, CA, USA). For the RNA-Seq data analysis Tophat 2.0.13, bowtie 2.1.0 , samtool 0.1.7 and cufflinks 1.3.0 were used. The rn5-bowtie2 index was generated with the command 'bowtie2-build rn5.fa rn5'. The 'rn5.fa'-file was downloaded from the UCSC genome browser. The mm10-bowtie2 index was downloaded from http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml. RefSeq geneTracks and GTF-files for the rn5 and mm10 genome assembly were downloaded from UCSC genome browser. Common gene ids in the GTF-files were matched to individual transcript_ids using the corresponding official symbols obtained from the geneTracks files. Conclusions: Our study illustrates the nature of the different transcripts in acute rat hippocampal slices after LTP 90 minutes was induced using field electrophysiology and compares them with control, unstimulated slices. Thius allowed us to identify wich genes are modulated in the hippocampus in order to promote and sustain LTP.

2018-10-02 | GSE120712 | GEO

Expression data from placenta harvested from WT and Pth-null fetuses treated 90 minutes prior with saline or PTH (1-84)

Project description:Parathyroid hormone (PTH) plays an essential role in regulating calcium and bone homeostasis in the adult, but whether PTH is required at all for regulating fetal-placental mineral homeostasis is uncertain. To address this we treated Pth-null mice in utero with 1 nmol PTH (1-84) or saline and examined placental calcium transfer 90 minutes later. It was found that placental calcium transfer increased in Pth-null fetuses treated with PTH as compared to Pth-null fetuses treated with saline. Subsequently, to determine the effect of PTH treatment on placental gene expression, in a separate experiment, 90 minutes after the fetal injections the placentas were removed for subsequent RNA extraction and microarray analysis.

2010-03-01 | GSE16983 | GEO

Transcription profiling of yeast to determine polysome-associated mRNAs after 90 minutes of mRNA mistranslation induction

Project description:Polysome-associated transcriptome analysis in cells after 90 minutes of mistranslation induction comparing with a pool of mRNAs existent in cells without induction at several time points (Reference)

2010-02-01 | E-MTAB-166 | biostudies-arrayexpress

mRNA sequencing analysis of transcripts from CA1 regions 90 minutes after Long-Term Potentiation was induced

Project description:mRNA sequencing analysis of transcripts from CA1 regions 90 minutes after Long-Term Potentiation was induced

| PRJNA494238 | ENA

Flavobacterium sp. 90 strain:90

Project description:Flavobacterium sp. 90 strain:90 Genome sequencing and assembly

| PRJNA442003 | ENA

RNAseq of camta3/dsc1/dsc2 after elf18 or mock treatment, against wt control, at 10, 30 and 90 minutes

Project description:The general stress response is a major transcriptional reprogramming in elicitor response. CAMTA3 is a key regulator of the general stress response, and is guarded by Dominant Suppressor of CAMTA3 1 and DSC2. Here we assay transcriptional response to elf18, an elicitor, at 10 (primary response), 30 (major GSR) and 90 (beginning to be immunity-specific responses) minutes, in wt and in a line mutated in camta3/dsc1/dsc2, thus losing the GSR with only partial autoimmune activation.

2022-12-31 | E-MTAB-10210 | biostudies-arrayexpress

Transcriptome analysis of IMR-90 human fibroblasts following oncogene-induced and replicative senescence

Project description:By transcriptome analysis of IMR-90 human fibroblasts following oncogene-induced senescence (OIS) and replicative senescence (RS), we identified commonly regulated genes in both conditions.

2017-03-30 | GSE86546 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data