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Differential expression of genes associated with inflammation in KC (Kupffer cells) after partial hepatoctomy

ABSTRACT: The 70% resection promotes migration of Ly6C+CD11b+ monocytes/macrophages to the remnant liver accompanied by a reduction in its CD206+ macrophage content. Macrophage proliferation within the liver reaches maximum on day 3 after the surgery. Transcriptomic profiles of macrophages isolated from the regenerating livers reveal chemokine signaling signatures in combination with indications of the involvement in specific metabolic pathways rather than inflammatory effects or morphogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE159197 | GEO | 2020/10/08

REPOSITORIES: GEO

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microRNA profiles in KC (Kupffer cells) after partial hepatoctomy

Project description:The 70% resection promotes migration of Ly6C+CD11b+ monocytes/macrophages to the remnant liver accompanied by a reduction in its CD206+ macrophage content. mRNA and microRNA expression profiles were studied by NanoString technology and verified by a reverse transcription-based qPCR assay. Corresponding microtranscriptomic profiles cannot be unambiguously defined as pro- or anti-inflammatory. Their typical features include elevated expression of leukocyte chemoattractant factors, and many of the differentially expressed sequences are related to the control of cell growth and metabolic processes in the liver.

2020-10-08 | GSE159198 | GEO

microRNA profiles in KC (Kupffer cells) and MNCs (blood monocytes):

Project description:As a rule, mammalian organs contain a mixed population of macrophages from different sources of origin. It is assumed that the self-sustaining resident macrophages and the monocyte-derived transient macrophages differ by their functional properties and their roles in repair processes . The analysis of the functional features of different populations of macrophages is of significant practical importance, since macrophages are considered as therapeutic targets in various pathological conditions and as prospective basis for cell therapy products. The aim of this study was to compare microRNA profiles of the two alternative macrophage species exemplified, respectively, by Kupffer cells of the liver and monocytes of the blood.

2020-05-19 | GSE150808 | GEO

High throughput quantitative whole transcriptome analysis of individual macrophages 7 days post-pneumonectomy in a B6 CSF1R-GFP mouse

Project description:Using fluorescence activated cell sorting, we isolated CD45+, CSF1R-GFP+, F4/80+, Ly6G- mouse lung monocytes and macrophages at 7 days after pneumonectomy procedure. We then used microfluidic single cell RNA-sequencing to transcriptional profile unique myeloid subsets. Using the pneumonectomy dataset, we identified 6 cell groups and 4 gene groups that marked several regenerative macrophage subsets including CCR2+, Ly6C+ monocytes and CD206+, Chil3+ M2-like macrophages.

2017-06-19 | GSE96104 | GEO

Differential expression of genes associated with inflammation in KC (Kupffer cells) and MNCs (blood monocytes):

Project description:As a rule, mammalian organs contain a mixed population of macrophages from different sources of origin. It is assumed that the self-sustaining resident macrophages and the monocyte-derived transient macrophages differ by their functional properties and their roles in repair processes . The analysis of the functional features of different populations of macrophages is of significant practical importance, since macrophages are considered as therapeutic targets in various pathological conditions and as prospective basis for cell therapy products. The aim of this study was to compare gene expression profiles of the two alternative macrophage species exemplified, respectively, by Kupffer cells of the liver and monocytes of the blood.

2020-05-20 | GSE150878 | GEO

COMPARATIVE ANALYSIS OF THE PROTEOME OF KUPFFER CELLS AND MONOCYTES

Project description:Macrophage population in most mammalian organs consists of cells of different origin, with the exception of the central nervous system and the liver, where macrophages of monocytic origin are almost completely absent. The reasons for such distribution and the phenomenon of coexistence of the two separate macrophage lineages with different origin in mammals remain poorly understood. In present study we compared Kupffer cells and monocytes by the immunophenotype, gene expression profile, proteome and pool of mircoRNA. Observed differences do not allow to consider the resident liver macrophages as purely M2 macrophages or monocytes have purely M1 features. Two populations of macrophages of monocytic origin and resident macrophages have fundamentally different roles in maintaining homeostasis. Monocytic macrophages are involved in the regulation of inflammation, and resident macrophages are involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis). However, if other indicators would be considered as markers of macrophage activity, it is worth noting that Kupffer cells possess some features of M2 macrophages. This is indicated by their expression profile of let-7b/c/d/e miRNAs, a high content of proteins associated with oxidative phosphorylation, as well as an increased level of synthesis of Arg1, IL10.

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Infection history imprints prolonged changes to the Kupffer cell epigenome, transcriptome and function of Kupffer cells

Project description:Background and aims: Macrophages are highly versatile innate phagocytes present in most organs of the body and perform various homeostatic and immunological functions. Macrophages are classically known for their important roles in compacting pathogens. Whereas, increasing evidence supports that exposure to infections invokes prolonged changes to tissue-resident macrophages, it remains unclear whether this occurs to liver macrophages. Methods: Here, we utilized a model of parasitic infection invoked by the protozoan parasite Trypanosoma brucei brucei to investigate whether infection history can durably reshape hepatic macrophage identity and function. Employing a combination of genetic lineage-tracing, single cell transcriptomics, epigenomic analysis, and functional assays, we studied the alterations to the liver macrophage compartment during and after the resolution of infection. Results: We show that the infection alters the composition of liver-resident macrophages, leading to the infiltration of monocytes that differentiate into various infection-associated macrophage populations with divergent transcriptomic profiles. Whereas infection-associated macrophages disappear post-resolution of infection, monocyte-derived macrophages assuming the Kupffer cell (KC)-like profile engraft in the liver and co-existed with embryonic KCs in the long-term. Remarkably, the prior exposure to infection imprinted a specific transcriptional program on post-resolution KCs that was independent of cell ontogeny. This reprogramming of KCs was driven by an epigenetic remodeling of KC chromatin landscapes and associated with altered KC function and was associated with increased resiliance to subsequent bacterial infection. Conclusion: Our study demonstrates that prior exposure to a parasitic infection can reshape the identity and function of the liver macrophage pool in the long-term.

2024-08-12 | GSE266225 | GEO

Identification of a macrophage PPARÎ³-GDF3 regulatory axis controlling muscle regeneration

Project description:Muscle injury was elicited by cardiotoxin injection into the tibialis anterior muscle. Macrophages were isolated 2 days post-injury from the regenerating muscle. We used microarray to obtain global gene expression data of muscle-derived tissue macrophage subsets. Tissue macrophages were collected from regenerating muscle samples of three animals, Ly6C+ F4/80low and Ly6C- F4/80high macrophage subsets were sorted. The global gene expression patterns of distinct macrophage subsets were analyzed on Affymetrix microarrays.

2016-10-24 | E-GEOD-71155 | biostudies-arrayexpress

Tissue macrophage subsets derived from regenerating muscle

2014-01-02 | E-GEOD-44057 | biostudies-arrayexpress

Single-cell RNAseq analysis (10X Genomics Chromium) of cMAF- and Mafb-deficent lung monocytes and interstium macrophages

Project description:Lung interstitium macrophages (IMs) are non-alveolar resident tissue macrophages which contribute to the lung homeostasis. These cells were reported to be heterogeneous by our group and other teams, which contains two main distinct subpopulations: CD206+ IMs and CD206- IMs. However, the exact origin of IMs and the transcriptional programs that regulate IM differentiation remains unclear. In recent report, we analyzed the refilled IMs in the course of time after induced IM depletion with single-cell RNA sequencing (10X Genomics Chromium) and bulk RNA sequencing. The lung IMs and monocytes from either Lyz2-Cre Mafflox/flox mice (cMAF-KO), Lyz2-Cre Mafbflox/flox (MAFb-KO), Lyz2-Cre Mafflox/flox Mafbflox/flox (dKO) or control litermate mice without floxp locus (Control) were analyzed and compared using single-cell RNA sequencing. All the main substs, i.e. Ly6C+ classical monocytes, Ly6C- patrolling monocytes, CD206+ IMs, CD206- IMs were found in control sample, while IMs are absent in both MAFb-KO and dKO sample accompied by a new intermediate population independent of Mafb expression. CMAF-KO sample show less impact in cell population composition with a lower freqency of CD206+ IM population.

2023-02-07 | GSE193891 | GEO

RNA sequencing of tumor associated macrophages and T cells in clear cell renal cell carcinoma

Project description:Tumor associated macrophages and T cells were characterized in a cohort of 73 ccRCC and 5 healthy donors using mass cytometry. Macrophage and T cell subsets of interest were identified and sorted directly from samples known to contain a high frequency of these subsets. CD8+ PD-1- T cells (n=6), exhausted CD8+ PD-1+ T cells (n=6), control macrophages CD14+ HLA-DR+ CD204- CD38- CD206- (n=11) and a population of TAM characterized as CD14+ HLA-DR+ CD204+ CD38+ CD206- (n=6) were isolated using FACS sorting.

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