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Quantification of transcriptional changes during in vivo mouse erythropoiesis by nascent RNA-sequencing

ABSTRACT: We isolated erythroid progenitor cells at various stages of differentiation from mouse fetal liver using flow cytometry. We metabolically labelled RNA from purified progenitor populations with 4-thiouridine (4sU) for 45 minutes in ex vivo culture, then extracted cellular RNA. Nascent RNA was biotinylated by reaction of the incorporated thiol groups with MTS-Biotin, then purified using streptavidin nanobeads and sequenced.

ORGANISM(S): Mus musculus

PROVIDER: GSE159229 | GEO | 2020/11/02

REPOSITORIES: GEO

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