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RTF1 and SPT6 stimulate transcription elongation by two distinct mechanisms

ABSTRACT: We have previously shown that the RNA polymerase II (Pol II)-DSIF complex associates with the PAF1 complex (PAF), RTF1 and SPT6 to form an activated elongation complex in vitro. Here we investigate the mechanisms that these factors use to stimulate Pol II elongation in vivo. We combine rapid factor depletion from human cells with genome-wide analyses of changes in RNA synthesis and occupancy with engaged Pol II. Whereas depletion of PAF subunits has little effect on transcription in vivo, depletion of RTF1 or SPT6 strongly compromises RNA synthesis, albeit in fundamentally different ways. RTF1 depletion decreases Pol II velocity, whereas SPT6 depletion impairs Pol II progression through nucleosomes. These results show that distinct transcription elongation factors stimulate Pol II velocity and Pol II progression through chromatin in vivo. Our results also provide evidence for two distinct barriers to elongation at the beginning of genes, the promoter-proximal pause site and the +1 nucleosome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE159633 | GEO | 2021/06/21

REPOSITORIES: GEO

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RTF1 and SPT6 stimulate transcription elongation by two distinct mechanisms

Project description:RTF1 and SPT6 stimulate transcription elongation by two distinct mechanisms

| PRJNA669878 | ENA

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