Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense: plp2 paraquat

ABSTRACT: af13_plp2 - plp2 paraquat - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE15973 | GEO | 2009/05/10

SECONDARY ACCESSION(S): PRJNA115555

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

af13_plp2 - plp2 paraquat - Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course

2009-05-05 | GSE15882 | GEO

Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense: plp2 paraquat

Project description:af13_plp2 - plp2 paraquat - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 10 uM Paraquat was infiltated in mesophyll of leaves with a syringe without needle. Leaf material was harvested at 0, 4 and 18 hours later. 3 plant genotypes were used (Col-0 ecotype) : - siPLP2 (RNAi-silenced) - pBIN (empty pBIN-transformed) - PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),time course 6 dye-swap - CATMA arrays

2009-05-09 | E-GEOD-15973 | biostudies-arrayexpress

af13_plp2 - plp2 paraquat - Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

2009-05-04 | E-GEOD-15882 | biostudies-arrayexpress

Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense: plp2 Botrytis cinerea

Project description:af13_plp2 - plp2 botrytis cinerea 2 - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 5000 spores of Botrytis were pipetted on 4 infection sites per ault leaf. Leaf material was harvested at 0 and 2 days later. 3 plant genotypes were used (Col-0 ecotype): siPLP2 (RNAi-silenced),pBIN (empty pBIN-transformed),PLP2OE (PLP2-overexpressors).

2009-05-10 | GSE15972 | GEO

plp2 bc 1-Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 2500 spores of Botrytis cinerea were pipetted on 4 infection sites per adult leaf. Leaf material was harvested at 0, 24 and 48 hours. 3 plant genotypes were used (Col-0) : PLP2- silenced (RNAi), control, PLP2-overexpressors. Keywords: normal vs rnai mutant comparison,time course,treated vs untreated comparison

2009-05-05 | GSE15880 | GEO

Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense: plp2 Botrytis cinerea

2009-05-09 | E-GEOD-15972 | biostudies-arrayexpress

plp2 botrytis cinerea 2-Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 5000 spores of Botrytis were pipetted on 4 infection sites per ault leaf. Leaf material was harvested at 0,1,2,3 days later. 3 plant genotypes were used (Col-0 ecotype): siPLP2 (RNAi-silenced),pBIN (empty pBIN-transformed),PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),normal vs rnai mutant comparaison,time course

2009-05-05 | GSE15881 | GEO

plp2 bc 1-Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 2500 spores of Botrytis cinerea were pipetted on 4 infection sites per adult leaf. Leaf material was harvested at 0, 24 and 48 hours. 3 plant genotypes were used (Col-0) : PLP2- silenced (RNAi), control, PLP2-overexpressors. Keywords: normal vs rnai mutant comparison,time course,treated vs untreated comparison 5 dye-swap - CATMA arrays

2009-05-04 | E-GEOD-15880 | biostudies-arrayexpress

plp2 botrytis cinerea 2-Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

Project description:Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 5000 spores of Botrytis were pipetted on 4 infection sites per ault leaf. Leaf material was harvested at 0,1,2,3 days later. 3 plant genotypes were used (Col-0 ecotype): siPLP2 (RNAi-silenced),pBIN (empty pBIN-transformed),PLP2OE (PLP2-overexpressors). Keywords: gene knock in (transgenic),normal vs rnai mutant comparaison,time course 4 dye-swap - CATMA arrays

2009-05-04 | E-GEOD-15881 | biostudies-arrayexpress

Characterization of 35S::TT2 and 35S::MYB5 overexpressors RUN2

Project description:Characterization of the impact of TT2 or MYB5 overexpression on gene expression. Col 0 plants were compared to 35S::TT2 and 35S::MYB5 overexpressors in control conditions

2021-07-15 | GSE171922 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data