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ChAP-seq analysis to identify GoxR binding sites

ABSTRACT: Gene expression in the obligatory aerobic acetic acid bacterium Gluconobacter oxydans was shown to respond to oxygen limitation, but the regulators involved are unknown. In this study, we analyzed the function of a transcriptional regulator named GoxR, which belongs to the FNR family. Here, we applied ChAP-seq analysis with a strep-tagged GoxR version to identify binding sites of this regulator in the genome of G. oxydans.

ORGANISM(S): Gluconobacter oxydans

PROVIDER: GSE159734 | GEO | 2021/03/11

REPOSITORIES: GEO

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Comparison of global gene expression in the Gluconobacter oxydans DgoxR mutant and the parental strain by RNA-Seq

Project description:Gene expression in the obligatory aerobic acetic acid bacterium Gluconobacter oxydans was shown to respond to oxygen limitation, but the regulators involved are unknown. In this study, we analyzed the function of a transcriptional regulator named GoxR, which belongs to the FNR family. Here, we aplied RNAseq to identify target genes of GoxR by comparing genes expression of a delta-goxR and its parental strain after 20 min of oxygen limitation.

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