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GABA-receptive microglia selectively sculpt developing inhibitory circuits

ABSTRACT: Microglia, the resident immune cells of the brain, have emerged as crucial regulators of synaptic refinement and therefore wiring precision. However, whether the remodeling of distinct synapses during development is mediated by specialized microglia is unknown. Here, using in vivo two-photon imaging, we show that GABA-receptive microglia selectively interact with inhibitory synapses during a critical window of mouse postnatal development. GABA initiates a transcriptional synapse remodeling program within these specialized microglia, which in turn sculpt inhibitory connectivity without impacting excitatory synapses. Ablation of GABAB receptors within microglia impairs this process and leads to stereotyped repetitive behavior and hyperactivity. These findings demonstrate that distinct microglia differentially engage with specific synapse types during development.

ORGANISM(S): Mus musculus

PROVIDER: GSE159947 | GEO | 2021/11/09

REPOSITORIES: GEO

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GABA-receptive microglia selectively sculpt developing inhibitory circuits

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