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Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [i-CLIP]

ABSTRACT: We show that the RNA-binding protein CSDE1/UNR promotes oncogene-induced senescence (OIS) in primary mouse keratynocytes challenged by over-expression of H-RASv12. Depletion of CSDE1 leads to senescence bypass, cell immortalization and tumor formation. Combining individual nucleotide cross-linking and immunoprecipitation (iCLIP), RNA-seq and polysome profiling followed by functional studies, we identify targets regulated by CSDE1 and uncover the downstream molecular mechanisms.

ORGANISM(S): Mus musculus

PROVIDER: GSE159997 | GEO | 2022/01/11

REPOSITORIES: GEO

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