Transcriptomics

Dataset Information

0

Single cell hibernation cultures identify molecules dispensable for haematopoietic stem cell self-renewal


ABSTRACT: Advances in the isolation and gene expression profiling of single haematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs (LT-HSCs) can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day culture system that removes single LT-HSCs from their native bone marrow microenvironment without compromising their functional potential. LT-HSCs are maintained as single cells and retain full HSC function compared to freshly isolated LT-HSCs with respect to divisional kinetics, colony forming cell potential (73.9%), and transplantation into primary (53%) and secondary (44%) recipients. Comparison of molecular profiles of single hibernating LT-HSCs to freshly isolated counterparts showed a high degree of similarity, but also identified key factors dispensable for LT-HSC function in transplantation assays including members of the AP1 complex (Jun, Fos, and Ncor2), Sult1a1 and Cish.

ORGANISM(S): Mus musculus

PROVIDER: GSE160131 | GEO | 2021/04/06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-07-23 | GSE222943 | GEO
2024-07-23 | GSE222942 | GEO
2024-07-23 | GSE222941 | GEO
2016-03-18 | GSE77477 | GEO
2019-09-23 | GSE115908 | GEO
2024-04-25 | GSE265789 | GEO
2019-11-10 | GSE130504 | GEO
2015-02-20 | E-GEOD-58299 | biostudies-arrayexpress
2022-11-01 | GSE165695 | GEO
2013-07-17 | E-GEOD-48539 | biostudies-arrayexpress