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MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE160254 | GEO | 2021/04/14

REPOSITORIES: GEO

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MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity.

Project description:MLL3 is a histone H3K4 methyltransferase that is frequently mutated in cancer, but the underlying molecular mechanisms remain elusive. Here, we found that MLL3 depletion by CRISPR/sgRNA significantly enhanced cell migration, but did not elevate the proliferation rate of cancer cells. Through RNA-Seq and ChIP-Seq approaches, we identified TNS3 as the potential target gene for MLL3. MLL3 depletion caused downregulation of H3K4me1 and H3K27ac on an enhancer ~ 7 kb ahead of TNS3. 3C assay indicated the identified enhancer interacts with TNS3 promoter and repression of enhancer activity by dCas9-KRAB system impaired TNS3 expression. Exogenous expression of TNS3 in MLL3 deficient cells completely blocked the enhanced cell migration phenotype. Taken together, our study revealed a novel mechanism for MLL3 in suppressing cancer, which may provide novel targets for diagnosis or drug development.

| S-EPMC8024252 | biostudies-literature

MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [RNA-seq]

Project description:MLL3 is a histone H3K4 methyltransferase which is frequently mutated in cancer, but the underlying molecular mechanisms remain elusive. Here, we found that MLL3 knockout by CRISPR/sgRNA did not elevate proliferation rate of cancer cells, but significantly enhanced cell migration. Through RNA-Seq and ChIP-Seq approaches, we identified TNS3 as the potential target gene for MLL3. MLL3 depletion caused down regulation of H3K4me1 and H3K27ac on an enhancer ~ 8 kb ahead of TNS3. 3C assay indicated the identified enhancer interacts with TNS3 promoter, and repression of enhancer with dCas9-KRAB system impaired TNS3 expression. Exogenous expression of TNS3 in MLL3 deficient cells completely blocked the enhanced cell migration phenotype. Taken together, our study revealed a novel mechanism for MLL3 in suppressing cancer, which may provide novel targets for diagnosis or drug development.

2021-04-14 | GSE160253 | GEO

MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [ChIP-seq]

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MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity

Project description:MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity

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MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [ChIP-seq]

Project description:MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [ChIP-seq]

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MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [RNA-seq]

Project description:MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [RNA-seq]

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Mll3 suppresses tumorigenesis by activating the Ink4a/Arf locus

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-08-01 | GSE85055 | GEO

Sox2 suppresses gastric tumorigenesis in mice

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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SIRT6 suppresses pancreatic cancer through control of Lin28b

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SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity.

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