Memory CD8+ T cells mediate early pathogen-specific protection through localized delivery of antigen-dependent chemokines and IFNgamma to clusters of inflammatory monocytes
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ABSTRACT: While cognate antigen-driven clonal expansion is a cardinal feature of memory CD8+ T cell effective response, inflammatory cytokines also orchestrates their rapid activation and production of IFNᵧ, contributing to innate mechanisms of protection. Despite antigen-independent secretion, IFNᵧ accounts for a large part of antigen-dependent protection in a multitude of infections, yet how cognate antigen enables memory CD8+ T cell-derived IFNᵧ to achieve host protection remains unknown. Herein, using distinct models of immunization, we establish that cognate antigen (Ag) recognition by memory CD8+ T cells on conventional CD11chi dendritic cells initiates their coordinated production of a burst of chemokines (CCL3, CCL4, XCL1) under the transcriptional control of IRF4. Using intravital microscopy imaging, we further reveal that memory CD8+ T cells undergo Ag-mediated arrest in splenic red pulp clusters of CCR2+ monocytes to deliver them with IFNᵧ- and Ag-dependent chemokine-potentiating microbicidal activities. These results establish that effective memory CD8+ T cell responses require coordinated stepwise processes that quickly restrict pathogen growth and optimize the local delivery of effector molecules before clonal expansion occurs.
ORGANISM(S): Mus musculus
PROVIDER: GSE160280 | GEO | 2020/10/29
REPOSITORIES: GEO
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