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Scalable pooled CRISPR screens with single cell chromatin accessibility profiling.

ABSTRACT: A scalable, cost-effective method that combines CRISPR perturbations with a single-cell indexing assay for transposase-accessible chromatin (CRISPR-sciATAC). This method links genome-wide chromatin accessibility to genetic perturbations through simultaneous capture of ATAC-seq fragments and CRISPR guide RNAs from single cells using a 96-well plate combinatorial indexing approach.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE161002 | GEO | 2020/11/07

REPOSITORIES: GEO

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