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Vitamin D Receptor binding and chromatin accessibility in mouse duodenal crypt and villus and colon epithelium

ABSTRACT: VDR is critical for Ca++ homeostasis. While its role in this process within the intestine has primarily been focused to the duodenal epithelium, we have uncovered a compensatory role for VDR in the colon in mediating Ca++ uptake. Here, we compare VDR genomic binding properties in the colon and duodenum using ChIP-seq. We also examine the chromatin accessibility between these intestinal regions.

ORGANISM(S): Mus musculus

PROVIDER: GSE161038 | GEO | 2023/11/06

REPOSITORIES: GEO

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Vitamin D Receptor binding and chromatin accessibility in mouse duodenal crypt and villus and colon epithelium

Project description:Vitamin D Receptor binding and chromatin accessibility in mouse duodenal crypt and villus and colon epithelium

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Apolipoprotein A4 Defines the Villus-Crypt Border in Duodenal Specimens for Celiac Disease Morphometry.

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Differential gene expression in Lrig1null duodenal tumors compared to normal duodenum

Project description:Leucine-rich repeats and immunoglobulin-like domains 1 (Lrig1) is a pan-ErbB negative regulator and intestinal stem cell marker downregulated in many malignancies. Over 91% Lrig1-CreERT2/CreERT2 (Lrig1-/-) mice developed duodenal adenomas, providing the first in vivo evidence Lrig1 acts as a tumor suppressor. We thus characterize the differential expressing transcripts in these duodenal adenomas to explore the pathegenesis. Elevated expression of the Egfr ligands was detected in adenomas compared to adjacent normal tissue. These adenomas also expressed the gastric-specific genes Gastrokine1 and Mucin5ac, indicating gastric metaplasia. In this dataset, we include the expression data obtained from proximal adenomas as well as adjacent normal tissue and wildtype proximal duodenum. These data are used to obtain 679 upregulated and 874 downregulated transcripts in tumors. Total RNA from matched duodenal tumors or adjacent normal or wildtype duodenum was isolated with Rneasy (with Dnase digestion) and used to probe the Affymetrix Mouse Gene 1.0 ST chip; the probe was prepared with Ambion Expression Target preparation Kit P/N 4411974.

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Differential gene expression in Lrig1null duodenal tumors compared to normal duodenum

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Transcription profiling of human duodenal mucosa samples taken from subjects after ingesting a Lactobacillus plantarum WCFS1 supplement for two weeks

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Endoscopic Placement of Metal Stents in Treating Patients With Cancer- Related Duodenal Obstruction

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