Transcriptomics

Dataset Information

0

Next Generation Sequencing Facilitates Quantitative Analysis of prostate cancer cell line LNCaP Transcriptomes


ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived NGF-overexpressed LNCaP cells transcriptome profiling (RNA-seq) to microarray methods and to evaluate NGF signature for optimal high-throughput data analysis. Method: LNCaP/EV and LNCaP/NGF cells were cultured in RPMI 1640 medium supplemented with 10% FBS. An RNeasy Midi Kit (Qiagen, Redwood City, CA, USA) was used for total RNA isolation. The cDNA library was prepared by KAPA mRNA HyperPrep Kits (Roche) and sequenced using the NovaSeqTM 6000 sequencing system (Illumina, San Diego, CA, USA) in 300~400-bp paired-end reads. Results: Here we show that an androgen-deprivation therapy (ADT)-stimulated transcription factor, ZBTB46, upregulated NGF via ZBTB46 mediated-transcriptional activation of NGF. Our results reveal a role of the NGF in the development of NEPC that is linked to ZBTB46 upregulation. Conclusion: Our study provides evidence that the ZBTB46-NGF axis has potential to be considered as a therapeutic target to impair NEPC progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE161070 | GEO | 2021/01/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-05-31 | GSE26114 | GEO
2022-01-05 | GSE178408 | GEO
2015-12-01 | GSE75471 | GEO
2012-12-10 | GSE37598 | GEO
2013-04-01 | GSE43383 | GEO
2020-06-09 | GSE152023 | GEO
2004-06-01 | GSE762 | GEO
2012-05-30 | GSE35429 | GEO
2007-09-25 | E-GEOD-762 | biostudies-arrayexpress
2020-08-12 | GSE136313 | GEO