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Functional genomic analyses on the role of Kr-h1 in the 20E/JH3 pathways in Harpegnathos ants

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Harpegnathos saltator

PROVIDER: GSE161207 | GEO | 2021/11/04

REPOSITORIES: GEO

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Functional genomic analyses on the role of Kr-h1 in the 20E/JH3 pathways in Harpegnathos ants

Project description:Functional genomic analyses on the role of Kr-h1 in the 20E/JH3 pathways in Harpegnathos ants

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Genomic comparison of the ants Camponotus floridanus and Harpegnathos saltator.

Project description:The organized societies of ants include short-lived worker castes displaying specialized behavior and morphology and long-lived queens dedicated to reproduction. We sequenced and compared the genomes of two socially divergent ant species: Camponotus floridanus and Harpegnathos saltator. Both genomes contained high amounts of CpG, despite the presence of DNA methylation, which in non-Hymenoptera correlates with CpG depletion. Comparison of gene expression in different castes identified up-regulation of telomerase and sirtuin deacetylases in longer-lived H. saltator reproductives, caste-specific expression of microRNAs and SMYD histone methyltransferases, and differential regulation of genes implicated in neuronal function and chemical communication. Our findings provide clues on the molecular differences between castes in these two ants and establish a new experimental model to study epigenetics in aging and behavior.

| S-EPMC3772619 | biostudies-literature

ChIP-seq for Kr-h1 in Harpegnathos brains

Project description:We performed ChIP-seq with a new Kr-h1 antibody in the central brains of in established gamergates (day 120) and age-matched workers

2021-11-04 | GSE161206 | GEO

RNA-seq in Harpegnathos central brains after Kr-h1 knockdown

Project description:Central brain RNA-seq after control and Kr-h1 knockdown in d10 worker or gamergate.

2021-11-04 | GSE161205 | GEO

RNA-seq in Harpegnathos ovaries after Kr-h1 knockdown in the brain

Project description:We injected mature (4-months-old) workers and gamergates with siRNAs against Kr-h1 or, as a control, GFP and performed RNA-seq on their ovaries 24 h later

2021-11-04 | GSE180308 | GEO

ChIP-seq for Kr-h1 in Harpegnathos brains

Project description:ChIP-seq for Kr-h1 in Harpegnathos brains

| PRJNA675872 | ENA

High-throughput sequencing analyses of two ants: Camponotus floridanus and Harpegnathos saltator

Project description:This SuperSeries is composed of the following subset Series: GSE22678: Transcriptome sequencing and analysis of two ants: Camponotus floridanus and Harpegnathos saltator GSE22679: Small RNA sequencing and analysis of two ants: Camponotus floridanus and Harpegnathos saltator Refer to individual Series

2010-08-26 | E-GEOD-22680 | biostudies-arrayexpress

High-throughput sequencing analyses of two ants: Camponotus floridanus and Harpegnathos saltator

Project description:This SuperSeries is composed of the SubSeries listed below.

2010-08-27 | GSE22680 | GEO

20E-mediated regulation of BmKr-h1 by BmKRP promotes oocyte maturation.

Project description:BackgroundKrüppel homolog 1 (Kr-h1) is a critical transcription factor for juvenile hormone (JH) signaling, known to play a key role in regulating metamorphosis and adult reproduction in insects. Kr-h1 can also be induced by molting hormone 20-hydroxyecdysone (20E), however, the underlying mechanism of 20E-induced Kr-h1 expression remains unclear. In the present study, we investigated the molecular mechanism of Kr-h1 induction by 20E in the reproductive system of a model lepidopteran insect, Bombyx mori.ResultsDevelopmental and tissue-specific expression analysis revealed that BmKr-h1 was highly expressed in ovaries during the late pupal and adult stages and the expression was induced by 20E. RNA interference (RNAi)-mediated depletion of BmKr-h1 in female pupae severely repressed the transcription of vitellogenin receptor (VgR), resulting in the reduction in vitellogenin (Vg) deposition in oocytes. BmKr-h1 specifically bound the Kr-h1 binding site (KBS) between - 2818 and - 2805 nt upstream of BmVgR and enhanced the transcription of BmVgR. A 20E cis-regulatory element (CRE) was identified in the promoter of BmKr-h1 and functionally verified using luciferase reporter assay, EMSA and DNA-ChIP. Using pull-down assays, we identified a novel transcription factor B. mori Kr-h1 regulatory protein (BmKRP) that specifically bound the BmKr-h1 CRE and activated its transcription. CRISPR/Cas9-mediated knockout of BmKRP in female pupae suppressed the transcription of BmKr-h1 and BmVgR, resulting in arrested oogenesis.ConclusionWe identified BmKRP as a new transcription factor mediating 20E regulation of B. mori oogenesis. Our data suggests that induction of BmKRP by 20E regulates BmKr-h1 expression, which in turn induces BmVgR expression to facilitate Vg uptake and oogenesis.

| S-EPMC7905918 | biostudies-literature

RNA-seq in Harpegnathos central brains after Kr-h1 knockdown

Project description:RNA-seq in Harpegnathos central brains after Kr-h1 knockdown

| PRJNA675871 | ENA