Project description:Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase.
Project description:Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase.
Project description:Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase.
Project description:Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase.
Project description:H4 lysine 20 dimethylation (H4K20me2) is the most abundant histone modification in vertebrate chromatin. It arises from sequential methylation of unmodified histone H4 proteins by the mono-methylating enzyme PR-SET7/KMT5A, followed by conversion to the dimethylated state by SUV4-20H (KMT5B/C) enzymes. We have blocked the deposition of this mark by depleting Xenopus embryos of SUV4-20H1/H2 methyltransferases. In the larval epidermis, this results in a severe loss of cilia in multiciliated cells (MCC), a key component of mucociliary epithelia. MCC precursor cells are correctly specified, amplify centrioles, but ultimately fail in ciliogenesis because of the perturbation of cytoplasmic processes. Genome-wide transcriptome profiling reveals that SUV4-20H1/H2-depleted ectodermal explants preferentially down-regulate the expression of several hundred ciliogenic genes. Further analysis demonstrated that knockdown of SUV4-20H1 alone is sufficient to generate the MCC phenotype and that its catalytic activity is needed for axoneme formation. Overexpression of the H4K20me1-specific histone demethylase PHF8/KDM7B also rescues the ciliogenic defect in a significant manner. Taken together, this indicates that the conversion of H4K20me1 to H4K20me2 by SUV4-20H1 is critical for the formation of cilia tufts.
