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RNA sequencing comparing human colon organoids transduced with Empty control vs. sgMGA (guide RNA targeting MGA)

ABSTRACT: The goal of this study was to compare expression profiles of normal colon organoids that have inactivated MGA compared to control organoids

ORGANISM(S): Homo sapiens

PROVIDER: GSE161543 | GEO | 2020/11/16

REPOSITORIES: GEO

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RNA sequencing comparing human colon organoids transduced with Empty control vs. sgMGA (guide RNA targeting MGA)

Project description:RNA sequencing comparing human colon organoids transduced with Empty control vs. sgMGA (guide RNA targeting MGA)

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CUT&RUN studies comparing occupancy of MAX, MGA, MYC and L3MBTL2 occupancy in Empty vector and sgMGA transduced A549 lung adenocarcinoma cells

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RNA sequencing comparing mouse Kras G12D lung tumors that have WT or inactivated Mga via CRISPR

Project description:The goal of this study was to compare expression profiles of mouse Kras G12D lung tumors that have inactivated MGA compared to controls

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M6 Mga+ vs Mga-

Project description:The transcriptional regulator Mga of Streptococcus pyogenes (the group A streptococcus, GAS) is known to directly activate several virulence genes important for colonization and immune evasion during exponential growth. Transcriptome analysis comparing two mga-1 serotypes (M1 SF370, M6 JRS4) and one mga-2 serotype (M4 GA40634) against their isogenic mga-inactivated strains uncovered a broader Mga regulon profile containing both activated and repressed genes with predicted functions primarily related to the uptake and metabolism of sugars. Although the divergent M1 and M4 Mga profiles were similar in size and content, the M6 JRS4 strain was clearly distinct, even from other M6 strains. Real-time RT-PCR and northern blot analysis validated our microarray results and confirmed that established core Mga regulon genes directly activated by Mga (emm, scpA, sof, fba) exhibited the highest activation levels across all strains tested. A novel ORF (Spy2036) encoding a cytosolic hypothetical protein was highly activated in all three serotypes and was called gene regulated by Mga or grm. Mga was shown to bind directly to Pgrm, which overlaps the Mga-regulated Psof in OF+ strains, suggesting that grm is part of the core Mga regulon and is able to activate two divergently transcribed genes from a single site in a class II background. Both class and serotype specific Mga-regulated genes, such as speB, were apparent. In fact, Mga activated speB as long as it was expressed in the wild type strain, although direct binding of Mga to the PspeB promoter could not be demonstrated. Thus, Mga is able to both directly and indirectly regulate genes shown to be important for virulence and the metabolic homeostasis of GAS. Keywords: Wild-type vs Mga-

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M4 Mga+ vs Mga-

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M1 Mga+ vs Mga-

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Project description:Mga

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Genomic occupancy of MYC and members of the PRC1.6 complex (MGA, E2F6, MAX, L3MBTL2) in MGA WT (Empty) and MGA depleted (sgMga) KrasG12D Trp53 -/- mouse lung cancer lines

Project description:Conventional crosslinked ChIP was performed on cell lines derived from control (Empty) and sgMga transduced tumors. We wanted to assess PRC1.6 member and MYC binding in the presence and absence of MGA.

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Phosphorylation events in the Multiple Gene Regulator of Group A Streptococcus (Mga) Significantly Influences Global Gene Expression and Virulence.

Project description:We sought understand the molecular mehcanism of gene regulation by Mga and its contribution to GAS pathogenesis in serotype M59 GAS through whole transcriptome analysis of strains with phosphorylation mimicking substitutions in key histidine residues of Mga. There were 6 strains analyzed, each in triplicate replicates: 1)wild-type GAS, 2)mga deletion strain 3)mga with alanine at H207 4)mga with aspartate at H207, 5) mga with alanine at H273 6)mga with aspartate at H273

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RNA sequencing comparing mouse lung cancer cell lines derived from Kras G12D Trp53 -/- and Kras G12D Trp53 -/- sgMga tumors

Project description:The goal of this study was to compare expression profiles of mouse Kras G12D Trp53 -/- lung cancer cells that have inactivated MGA compared to controls

2020-11-17 | GSE161606 | GEO

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