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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [TT-Seq]

ABSTRACT: The relationship between chromatin and transcription was investigated through condensin, a key regulator of higher order chromatin structure. We use rapid conditional condensin depletion in nocodozole arrested cells in budding yeast S. cerevisiae cells to study condensin’s role in transcriptional regulation. We use TT-Chemseq to assess global RNA levels through a S. pombe spike-in, and found that condensin depletion leads to upregulation of global transcription. We also found transcriptional changes in response to environmental stimuli are subdued by condensin depletion.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE161581 | GEO | 2021/05/21

REPOSITORIES: GEO

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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [RNA-Seq]

Project description:The relationship between chromatin and transcription was investigated through condensin, a key regulator of higher order chromatin structure. We use rapid conditional condensin depletion in cell cycle-staged budding yeast S. cerevisiae cells to study condensin’s role in transcriptional regulation. We observe a large number of small gene expression changes close to condensin binding sites

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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [TT-Seq]

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Condensin association with chromosomes depends on a DNA binding domain formed by its HEAT-repeat subunits

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