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RPL8 extensively regulates the expression and alternative splicing of cancer-related genes

ABSTRACT: Objective: In our study, we aimed to explore the molecular mechanism of RPL8 in tumors by analyzing over-expression of RPL8 in HeLa cells. Method: Two experimental groups and control groups were setup. The experimental groups adopted Hela cells transfected by RPL8 plasmid , and the control group used the Hela cells transfected by blank plasmid. The total mRNA of the cells were extracted after transfection for 48 hours and the expression level of mRNA was detected by using high-throughput sequencing technology. Then R Bioconductor software package edgeR was used to screen differentially expressed genes and TopHat2 was used to analyze the splice site of each sample. Finally, GO and KEGG analysis of differentially expressed genes and differential alternative splicing events were performed, and the top ten functional pathways were selected for display. Results: Overexpression of RPL8 produced a large number of differentially expressed genes and differential alternative splicing. Many of these differentially expressed genes and differential alternative splicing genes were enriched in tumor-related pathways, including inflammation, positive regulation of cell proliferation , Angiogenesis, etc. In addition, many of these differentially expressed genes have been shown to be closely related to the occurrence and development of tumors. Conclusion: RPL8 can widely regulate the expression and alternative splicing of tumor-related genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE161696 | GEO | 2024/01/03

REPOSITORIES: GEO

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