Transcriptomics

Dataset Information

0

RNA Sequencing Analysis of cultured macrophages harvested from the infarcted heart of WT mice and il21 receptor deficient mice (il21r-/-)


ABSTRACT: Ly6Clow macrophages promote scar formation and prevent early infarct expansion after myocardial infarction (MI). Although CD4+ T cells influence the regulation of Ly6Clow macrophages after MI, the mechanism remains largely unknown. Here, we focused on IL-21 and uncovered its physiological relevance in post-MI hearts. CD4+ T cells harvested from the infarcted heart produce IL-21 upon stimulation, and IL-21 receptor was expressed on Ly6Clo macrophages in the infarcted heart. The survival rate after MI was significantly improved in IL-21-deficient mice compared with WT mice. Moreover, transcriptome analysis of infarcted heart tissue demonstrated that inflammation was persistent in WT mice compared with IL-21-deficient mice. The number of neutrophils was significantly decreased, whereas the number of Ly6Clow macrophages was significantly increased in IL-21-deficient mice. Consistently, IL-21 enhanced the apoptosis of Ly6Clow macrophages. Furthermore, RNA-seq analysis of Ly6Chi and Ly6Clo macrophages stimulated with or without IL-21 for 24 hours revealed that IL-21 induces inflammatory responses in both Ly6Chi and Ly6Clo macrophages. Finally, the treatment with IL-21 receptor Fc protein significantly increased the survival after MI. Thus, the deletion of IL-21 improves survival after MI by preventing Ly6Clo macrophage apoptosis.

ORGANISM(S): Mus musculus

PROVIDER: GSE161868 | GEO | 2021/10/31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-09-27 | GSE115151 | GEO
2021-08-01 | GSE118861 | GEO
2010-06-20 | E-GEOD-18703 | biostudies-arrayexpress
| PRJNA473977 | ENA
2024-03-18 | GSE254202 | GEO
2022-12-31 | GSE214696 | GEO
2009-10-24 | GSE18703 | GEO
2022-12-31 | GSE190868 | GEO
2017-11-06 | GSE106471 | GEO
2017-11-06 | GSE106472 | GEO