Transcriptomics

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Transcriptional dissection of lateral root primordium cells


ABSTRACT: To specifically profile early stages of lateral root formation we used the marker pHB53:NLS-3xmCherry, which is only expressed in lateral root primordium cells. We isolated ~2,000 lateral root primordium cells from stages I to IV through Fluorescent Activated Cell Sorting, and profiled the transcriptome of 573 of these cells. As a LR primordium has ~6-10 cells at stage I and ~30-40 cells at stage IV, the number of sequenced cells would approximately cover 5-6 fold the total number of cells in the stages profiled.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE161970 | GEO | 2021/08/05

REPOSITORIES: GEO

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