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Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

ABSTRACT: An RIP experiment was performed in ARP1 cell following the instructions of the article. ~5-20 × 10^6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5μg of the antibody of interest (HNRNPA2B1/ILF3, Proteintech) and incubated with cell lysate supernatant overnight at 4°C.The beads containing immunoprecipitated RNA-protein complex were treated with 150μL of Proteinase K buffer to digest the proteins. Specific binding RNAs were isolated by using TRIzol.

ORGANISM(S): Homo sapiens

PROVIDER: GSE162303 | GEO | 2021/11/30

REPOSITORIES: GEO

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Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

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