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Vascular integrity is essential for maintaining tissue homeostasis I

ABSTRACT: Purpose: analyze the transcriptomic differences in PBS, LPS and LPS + Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS, LPS or LPS + Riociguat treatment via trachea instillation. 24h later, homogenize lung and extract mRNA Results: Riociguat can restore LPS-induced lung injury by improving immune environment

ORGANISM(S): Mus musculus

PROVIDER: GSE162328 | GEO | 2021/06/01

REPOSITORIES: GEO

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Vascular integrity is essential for maintaining tissue homeostasis

Project description:Purpose: analyze the transcriptomic differences in PBS and Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS or Riociguat treatment (i.g.). 24h later, homogenize lung and extract mRNA Results: Riociguat has merely effect on normal mice lung

2022-07-13 | GSE207868 | GEO

Vascular integrity is essential for maintaining tissue homeostasis II

Project description:Purpose: analyze the transcriptomic difference of endothelial cells and pericytes in PBS, LPS or LPS + Riociguat treated lungs Methods: Gucy1a1-EGFP mice were given PBS or LPS treatment via trachea instillation. Vehicle or Riociguat oral administation was performed at every 9 hours. 24h later, lungs were dissociated and endothelial cells and pericytes were isolated by flow cytometry, then directly processed to RNA extraction and library construction Results: Riociguat can restore LPS-induced lung injury by inhibiting pericytes activation and maintain vascular homostatsis

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Vascular integrity is essential for maintaining tissue homeostasis III

Project description:Purpose: analyze the differential changed endothelial cells and pericytes interacton in normal and diseased condition Methods: Pdgfrb-EGFP mice were given PBS or LPS treatment via trachea instillation. 24h later, dissociate lung and isolate endothelial cells and pericytes by flow cytometry, then directly processed to RNA extraction and library construction Results: LPS induced a great transcriptomic change in pericytes than in endothelial cells and NO-sGC pathway was impaired during LPS-induced lung injury

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