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Gene expression data of mRNA from longissimus muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

ABSTRACT: 47,845 probe-sets were screened, in the first part of this study pig embryos at 63 days post conception (dpc)

ORGANISM(S): Sus scrofa

PROVIDER: GSE162754 | GEO | 2021/08/31

REPOSITORIES: GEO

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Gene expression data of mRNA from liver sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

Project description:47,880 probe-sets were screened, in the first part of this study pig embryos at 63 days post conception (dpc)

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Gene expression data of micro RNA from skeletal muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

Project description:Gene expression data of micro RNA from skeletal muscle samples of F2 population based DL and Pi F2 63 days post conception (dpc).

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Gene expression data of mRNA from longissimus muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

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Gene expression data of mRNA from mesenchymal stem cells derived from two different sorts of synovial membrane of German Landrace (DL) and Angeln Saddleback (AS) pigs

Project description:47,845 probe-sets were screened, This study compared expression profiles of mesenchymal stem cells derived from the two breed DL and AS and then analyzed the expression profile genes between two different sorts of synovial membrane in each breed.

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Gene expression data of mRNA from liver sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

Project description:Gene expression data of mRNA from liver sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

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Gene expression data of micro RNA from skeletal muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

Project description:Gene expression data of micro RNA from skeletal muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds

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PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs.

Project description:Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting the swine industry worldwide. Genetic variation in host immunity has been considered as one of the potential determinants to improve the immunocompetence, thereby resistance to PRRS. Therefore, the present study aimed to investigate the breed difference in innate immune response to PRRSV vaccination between German Landrace (DL) and Pietrain (Pi) pigs. We analyzed microarray-based transcriptome profiles of peripheral blood mononuclear cells (PBMCs) collected before (0 h) and 24 h after PRRSV vaccination from purebred DL and Pi pigs with three biological replicates. In total 4,269 transcripts were identified to be differentially expressed in PBMCs in at least any of four tested contrast pairs (i.e. DL-24h vs. DL-0h, Pi-24h vs. Pi-0h, DL-0h vs. Pi-0h and DL-24h vs. Pi-24h). The number of vaccine-induced differentially expressed genes (DEGs) was much higher (2,459) in DL pigs than that of Pi pigs (291). After 24 h of PRRSV vaccination, 1,046 genes were differentially expressed in PMBCs of DL pigs compared to that of Pi (DL-24h vs. Pi-24h), indicating the breed differences in vaccine responsiveness. The top biological pathways significantly affected by DEGs of both breeds were linked to immune response functions. The network enrichment analysis identified ADAM17, STAT1, MMS19, RPA2, BAD, UCHL5 and APC as potential regulatory genes for the functional network of PRRSV vaccine response specific for DL; while FOXO3, IRF2, ADRBK1, FHL3, PPP2CB and NCOA6 were found to be the most potential hubs of Pi specific transcriptome network. In conclusion, our data provided insights of breed-specific host transcriptome responses to PRRSV vaccination which might contribute in better understanding of PPRS resistance in pigs.

| S-EPMC6752781 | biostudies-literature

Gene expression data of mRNA from skeletal muscle sample of pig DL and Pi breed

Project description:The skeletal muscle development in swine begins at embryogenesis. Simultaneously, 24,123 probe-sets were screened, analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development (35, 63, 91 dpc and adult stage).

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