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Enhancer RNAs make multivalent interactions with NELF to stimulate Pol II pause release [SHAPE]

ABSTRACT: A SHAPE-MaP structure probing experiment was performed on 40 eRNAs. The eRNA transcription start sites (TSSs) were identified by 5'-ExoSeq. The 5' end fragment (1-200 nucleotides) of each eRNA was cloned from mouse cortical neurons and the eRNAs were produced by in vitro transcription. Each eRNA was treated in one sample with DMSO (control) and in a second sample with the SHAPE reagent 1-Methyl-7-nitroisatoic anhydride (1M7). The 1M7 chemical will react preferentially with the ribose 2'OH of the flexible nucleotides in single stranded regions and the produced adduct will lead to mutations during reverse transcription.

ORGANISM(S): Mus musculus

PROVIDER: GSE163112 | GEO | 2022/03/08

REPOSITORIES: GEO

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Enhancer RNAs harness multivalent interactions to detach NELF from paused Pol II [GROSEQ, eCLIPSEQ, CHIPSEQ]

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Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

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Enhancer RNAs make multivalent interactions with NELF to stimulate Pol II pause release

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Enhancer RNAs make multivalent interactions with NELF to stimulate Pol II pause release [Exo-seq]

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Enhancer RNAs make multivalent interactions with NELF to stimulate Pol II pause release [SHAPE]

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