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Esophageal progenitor cell plasticity; a 3D organotypic model of epithelial regeneration

ABSTRACT: Epithelial stem cells are capable of remarkable plasticity, facilitating efficient regenerative responses following damage. Here, we present a versatile model of ectopic niche regeneration that provides evidence of a switch in epithelial stem cell identity as a response to foreign microenvironmental signals. A combination of 3D organotypic cultures, 3D confocal imaging and Next-Generation Sequencing reveal events of epithelial plasticity during the reepithelialization of denuded stroma. In this study we perform a temporal analysis that provides new insights on the mechanisms driving cell fate flexibility in adult epithelial stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE163218 | GEO | 2023/12/14

REPOSITORIES: GEO

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