Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RiboMethSeq of HCT116 cells treated with siTFIP11 or siDHX15

ABSTRACT: RiboMethSeq of HCT116 cells treated with siTFIP11 or siDHX15 to investigate the effect of TFIP11 or DHX15 knockdown on RNA methylation as part of a larger study on the function of TFIP11.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163369 | GEO | 2021/09/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RiboMethSeq of HCT116 cells treated with siCoilin

Project description:RiboMethSeq of HCT116 cells treated with siCoilin to investigate the effect of Coilin knockdown on RNA methylation as part of a larger study on the function of the protein TFIP11.

2021-09-22 | GSE179068 | GEO

RNA-Seq of HeLa cells treated with siDHX15

Project description:RNA-Seq of HeLa cells treated with siDHX15 or control siRNA to investigate the effect of DHX15 knockdown on mRNA as part of a larger study on the function of TFIP11 and DHX15.

2021-09-22 | GSE171446 | GEO

RNA-Seq of HeLa cells treated with siTFIP11

Project description:RNA-Seq of HeLa cells treated with siTFIP11 or control siRNA to investigate the effect of TFIP11 knockdown on mRNA as part of a larger study on the function of TFIP11.

2021-09-22 | GSE156388 | GEO

Small RNA-Seq of HeLa cells treated with siTFIP11

Project description:Small RNA-Seq of HeLa cells treated with siTFIP11 or control siRNA to investigate the effect of TFIP11 knockdown on small RNA (snRNA, snoRNA, etc.) as part of a larger study on the function of TFIP11.

2021-09-22 | GSE156389 | GEO

RiboMethSeq of HCT116 cells treated with siCoilin

Project description:RiboMethSeq of HCT116 cells treated with siCoilin

| PRJNA742051 | ENA

iCLIP of HeLa cells with anti-TFIP11 antibody

Project description:iCLIP of HeLa cells with anti-TFIP11 antibody to investigate RNA binding locations of TFIP11 as part of a larger study on the function of TFIP11.

2021-09-22 | GSE158589 | GEO

RiboMethSeq of HCT116 cells treated with siTFIP11 or siDHX15

Project description:RiboMethSeq of HCT116 cells treated with siTFIP11 or siDHX15

| PRJNA685837 | ENA

scRNA-seq of DAC-treated and mock-treated HCT116.

Project description:Epigenetic reprogramming using demethylating drugs is a promising approach for cancer therapy, but its efficacy is highly dependent on the dosing regimen. Low-dose treatment for a prolonged period shows a high therapeutic efficacy, despite its small demethylating effect. Here, we aimed to reveal the mechanisms of how such low-dose treatment shows high efficacy by focusing on epigenetic reprograming at the single-cell level. Single-cell RNA-sequencing of HCT116 cells treated with decitabine (DAC) revealed that up-regulated genes were highly variable at the single-cell level. To analyze functional consequences at the single-cell level, DAC-treated HCT116 cells were cloned. While only partial reduction of methylation levels was observed in bulk cells, complete demethylation of specific cancer-related genes was observed, depending upon clones. For example, p16 was completely demethylated in the H3-32 clone out of 9 clones, and this clone showed slower proliferation than other clones without demethylation. In addition, in this clone, the fraction of cells with tetraploid became much larger, indicating that cellular senescence was induced. These results showed that epigenetic reprogramming of specific cancer-related pathways at the single-cell level is likely to underlie the high efficacy of low-dose DNA demethylating therapy.

2021-04-10 | GSE171429 | GEO

Analysis of DNA methylation in TET1 depleted colorectal cancer HCT116 cells

Project description:We aimed to analyze the relationship between TET1 and aberrant CpG methylation in colorectal cancer (CRC). We established two stable TET1 knockdown clones and negative control clones of HCT116 cells, and carried out DNA methylation analysis with HumanMethylation450 BeadChip.

2017-01-01 | GSE84399 | GEO

DNA methylation inhibitor in HCT116

Project description:DNA methylation inhibitor in HCT116

| PRJNA422822 | ENA