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High-throughput mRNA sequencing of CLL cells containing an E571K XPO1 mutations

ABSTRACT: Purpose: Exportin 1 (XPO1/CRM1) is a key mediator of nuclear export with relevance to multiple cancers, including chronic lymphocytic leukemia (CLL). Whole exome sequencing has identified hot-spot somatic XPO1 point mutations which we found to disrupt highly conserved biophysical interactions in the NES-binding groove, conferring novel cargo-binding abilities and forcing cellular mis-localization of critical regulators. However, the pathogenic role played by change-in-function XPO1 mutations in CLL is not fully understood. Thus, we aimed to identify disrupted cellular pathways in response to the E571K XPO1 mutation, the mutation most frequently cited in CLL patients, via high-throughput RNA-sequencing Results & Conclusion: Multidimensional scaling (MDS) analysis of the top 1000 most variable genes demonstrated distinctly unique clustering along the first dimension for XPO1-E571K patient and XPO1-wt patient samples. This clustering pattern was driven by a number of genes that were either over- or under-expressed in XPO1-E571K patient cells, contributing to a unique gene expression pattern in patients with this mutation signature.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163370 | GEO | 2021/01/21

REPOSITORIES: GEO

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XPO1 amplification abrogates the effects of E571K XPO1 mutation in B-cell lymphoma

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High-throughput mRNA sequencing of CLL cells containing an E571K XPO1 mutations

Project description:High-throughput mRNA sequencing of CLL cells containing an E571K XPO1 mutations

| PRJNA685838 | ENA

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