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Transcriptome analysis of SARS-CoV-2 infected H522 human lung adenocarcinoma cells

ABSTRACT: Purpose: The goal of this study is to define the transcriptional response of H522 cells to SARS-CoV-2 infection. Methods:H522 cells were infected with SARS-CoV-2 at varying multiplicities of infection and samples processed for RNA-seq at 4, 24, 48, 72 and 96 hours post-infection. Results: We provide the transcriptomic and translatomic landscape of SARS-CoV-2-infected H522 cells from multiple RNA-seq libraries. We found that the robust transcriptional upregulation of numerous type I interferon and cell cycle associated genes in SARS-CoV-2-infected cells. Conclusions: Our study represents the detailed analysis of transcriptional responses of H522 cells to SARS-CoV-2 and demonstrates upregulation of key pathways that may paly a role in disease pathogenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163547 | GEO | 2021/01/31

REPOSITORIES: GEO

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Project description:Background: Type I interferons (IFNs) are essential to the clearance of viral diseases, in part by initiating upregulation of IFN regulated genes (IRGs). A clear distinction between genes upregulated directly by virus and genes upregulated by secondary IFN production has not been made. Here we investigated the genes regulated by IFN-a2b compared to the genes regulated by SARS-CoV infection in ferrets. Methods: We characterized early host immune responses in peripheral blood and lung necropsies of ferrets injected with IFN-a2b or infected with SARS-CoV/Tor 2 strain, using microarray analysis on the Affymetrix platform. Results: We identified a common IRG signature that was upregulated in both SARS-CoV infected ferrets as well as in ferrets injected with IFN-a2b. We also identified unique patterns of gene expression for leukocyte activation, cell adhesion and complement pathways between IFN-a2b injection and SARS-CoV infection. Conclusions: Our results define the effects of IFN-a2b on the immune system of ferrets highlighting genes regulated by IFN during SARS-CoV infection. We have shown the similarities and differences of top funcional gene groups as well as pathways that play key roles in early immune responses in ferrets in response to IFN-a2b or SARS-CoV. Key words: ferret, gene expression, SARS, interferon. Keywords: time course In experiments with IFN-a2b, for peripheral blood, 15 ferrets were randomly allocated to 3 groups: Day 0, 5 ferrets (no IFN injection), day 1, 6 ferrets (injected), and day 2, 4 ferrets (injected). For lung necropsies of injected ferrets with IFN-a2b, we used 12 ferrets in 3 groups: 4 ferrets, day 0 (no IFN injection), 4 ferrets, day 1 (injected) and 4 ferrets, day 2 (injected). Experimental groups for SARS-CoV infection was as follows: For peripheral blood, 3 and 4 ferrets for day 0 (no infection) and day 2 (infection) respectively. For lung neceropsies, a total of 9 ferrets in 3 groups, each with 3 replicates for day 0 (no infection), day 1 (infection) and day 2 (infection).

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Transcriptome analysis of SARS-CoV-2 infected H522 human lung adenocarcinoma cells

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